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产品描述：牛载脂蛋白A1(APOA1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOA1含量。APOA1是重要的载脂蛋白，在脂质代谢中意义重大。研究显示，其能促进胆固醇逆向转运，抑制动脉粥样硬化。还可稳定高密度脂蛋白结构，发挥抗炎症、抗氧化作用。对其深入研究有望为心血管疾病等的防治提供新思路。试剂盒检测范围为78 ng/mL-5000 ng/mL，本试剂盒适用于探索牛脂质代谢相关疾病机制、评估营养干预对脂蛋白合成的影响，或作为动物模型中动脉粥样硬化研究的工具，为畜牧养殖健康管理、动物疾病模型构建及基础医学研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：APOA1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
缩写：APOA1
Uniprot No.：P15497
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：78 ng/mL-5000 ng/mL
灵敏度：19.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine APOA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 90 | ||||
| Range % | 87-94 | |||||
| 1:2000 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:4000 | Average % | 93 | ||||
| Range % | 87-97 | |||||
| 1:8000 | Average % | 100 | ||||
| Range % | 96-103 | |||||
回收率：
| The recovery of bovine APOA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 82-87 | ||||
| EDTA plasma (n=4) | 89 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 50000.1400.1430.142 25000.2850.2910.288 12500.4820.5030.493 6250.8140.8210.818 3121.2531.2681.261 1561.6951.7091.702 782.0402.1342.087 02.4362.4582.447 | ||||||
货期：3-5 working days
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