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产品描述：犬载脂蛋白A1(Apo-A1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOA1含量。APOA1是一种位于细胞膜外的分泌蛋白，是高密度脂蛋白（HDL）的主要结构载脂蛋白，在逆向胆固醇转运途径中起关键作用。其研究机制涉及胆固醇代谢、抗炎、抗动脉粥样硬化等功能，是心血管疾病预防和治疗中的潜在靶点。试剂盒检测范围为28.12 ng/mL-1800 ng/mL，该产品适用于科研领域中对犬类脂代谢机制、心血管疾病模型构建、营养干预效果评估等研究，尤其在探究HDL功能与疾病相关性、药物或膳食成分对脂代谢影响的实验中具有广泛应用。组织匀浆兼容性设计还可支持特定器官或病理组织中Apo - A1的分布研究，为深入探索犬类脂质代谢的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：APOA1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
缩写：APOA1
Uniprot No.：P02648
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：28.12 ng/mL-1800 ng/mL
灵敏度：7 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog APOA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 93 | ||||
| Range % | 89-103 | |||||
| 1:2000 | Average % | 89 | ||||
| Range % | 85-95 | |||||
| 1:4000 | Average % | 95 | ||||
| Range % | 84-103 | |||||
| 1:8000 | Average % | 93 | ||||
| Range % | 83-98 | |||||
回收率：
| The recovery of dog APOA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 92-95 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average18000.1420.1450.1449000.2150.2290.2224500.3820.3670.3752250.5510.5580.555112.50.9730.9900.98256.251.6971.6231.66028.122.2872.3282.30802.6542.7612.708 | ||||||
货期：3-5 working days
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