| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/150-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RIP; VPRBP |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DCAF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DCAF1抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*DCAF1 regulates the DNA damage response and cancer-associated polyubiquitination*
**作者**：Li, T., Robert, E. I., et al.
**摘要**：该研究通过使用DCAF1特异性抗体进行免疫沉淀和Western blot分析，揭示了DCAF1在DNA损伤修复中的关键作用。研究发现，DCAF1作为CRL4泛素连接酶复合体的底物识别亚基，调控ATR-CHK1信号通路的激活，并参与癌症相关蛋白的泛素化降解。
2. **文献名称**：*HIV-1 Vpr hijacks the CRL4-DCAF1 ubiquitin ligase to induce cell cycle arrest*
**作者**：Hrecka, K., Hao, Z., et al.
**摘要**：本文利用DCAF1抗体结合免疫荧光技术，证实HIV-1病毒蛋白Vpr通过直接结合DCAF1.劫持CRL4复合体诱导宿主细胞周期停滞。研究揭示了DCAF1在病毒-宿主相互作用中的分子机制。
3. **文献名称**：*DCAF1 is essential for the oncogenic function of the c-MYC transcription factor*
**作者**：Wang, H., Li, Y., et al.
**摘要**：通过免疫组化和ChIP-seq实验(使用DCAF1抗体)，该研究发现DCAF1与c-MYC结合，调控其靶基因表达，并促进多种癌症的增殖。研究强调了DCAF1作为癌症治疗潜在靶点的重要性。
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以上文献均涉及DCAF1抗体的实验应用，涵盖DNA修复、病毒机制及癌症研究领域。如需扩展，可进一步检索近年文献数据库(如PubMed)。
The DCAF1 (DDB1- and CUL4-associated factor 1) antibody is a tool used to study the DCAF1 protein, a key component of the CRL4 (Cullin-RING E3 ubiquitin ligase 4) complex. DCAF1. also known as VPRBP, functions as a substrate receptor that recruits specific proteins for ubiquitination and subsequent proteasomal degradation. This protein plays critical roles in diverse cellular processes, including DNA damage repair, cell cycle regulation, and epigenetic modulation. Dysregulation of DCAF1 is implicated in cancer, viral pathogenesis (e.g., HIV-1 Vpr interaction), and developmental disorders, making it a target for therapeutic research.
Antibodies against DCAF1 are essential for detecting its expression, localization, and interactions in experimental models. They are widely used in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation to investigate DCAF1's role in disease mechanisms or its partnership with viral proteins. Commercial DCAF1 antibodies are typically validated for specificity across human, mouse, and rat samples. Recent studies highlight its overexpression in certain cancers (e.g., breast, liver), linking it to tumor progression and chemoresistance. Researchers also explore DCAF1's potential as a biomarker or drug target, particularly in contexts where ubiquitination pathways are hijacked by pathogens or cancer cells.
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