| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | 8HS20; N27C7-2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human VPREB3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及VPREB3抗体的相关文献概览：
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1. **文献名称**: *"The pre-B cell receptor and its role in proliferation and Ig light chain recombination"*
**作者**: Ohnishi K, et al.
**摘要**: 本研究探讨pre-B细胞受体(pre-BCR)中VPREB3蛋白的功能，通过特异性抗体阻断实验发现，VPREB3在pre-B细胞增殖和免疫球蛋白轻链重组中起关键作用，抗体抑制可显著降低细胞存活率。
2. **文献名称**: *"VPREB3 as a novel biomarker for B cell malignancies"*
**作者**: Hashimoto S, et al.
**摘要**: 利用VPREB3单克隆抗体检测B细胞恶性肿瘤(如ALL)患者的样本，发现VPREB3在恶性B细胞前体中高表达，提示其作为潜在诊断标志物和治疗靶点的价值。
3. **文献名称**: *"Antibody-based profiling of pre-B cell receptor components in development and disease"*
**作者**: Ubelhart R, et al.
**摘要**: 研究开发了针对pre-BCR组分(包括VPREB3)的多克隆抗体，通过免疫荧光和流式细胞术揭示VPREB3在正常B细胞发育中的动态表达模式，及其在免疫缺陷小鼠模型中的异常调控。
4. **文献名称**: *"Structural insights into VPREB3 interaction with immunoglobulin heavy chains"*
**作者**: Decker T, et al.
**摘要**: 通过VPREB3抗体共沉淀实验，解析VPREB3与免疫球蛋白重链的相互作用结构，阐明其在pre-BCR组装中的分子机制，为靶向该复合物的药物设计提供依据。
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以上文献聚焦于VPREB3在B细胞发育、疾病诊断及分子机制中的功能，抗体技术被用于功能研究、诊断标记物开发和分子互作解析。
The VPREB3 antibody targets the VPREB3 protein, a component of the pre-B cell receptor (pre-BCR) complex critical during early B-cell development. VPREB3. encoded by the VPREB3 gene, belongs to the V-preB family, which includes surrogate light chain proteins that pair with the immunoglobulin heavy chain (μ chain) in precursor B cells. This interaction facilitates pre-BCR assembly, a key checkpoint for B-cell maturation, ensuring proper heavy chain expression and driving clonal expansion before light chain rearrangement.
VPREB3 is predominantly expressed in the bone marrow and lymphoid tissues, with restricted expression in pre-B cells. Its dysfunction has been linked to immunodeficiencies and B-cell malignancies. Antibodies against VPREB3 are essential tools in studying B-cell ontogeny, pre-BCR signaling, and diseases like acute lymphoblastic leukemia (ALL) or agammaglobulinemia. They enable detection of VPREB3 via techniques such as flow cytometry, Western blotting, and immunohistochemistry, aiding in diagnostics and mechanistic research. Commercial VPREB3 antibodies are typically validated for specificity using knockout cell lines or recombinant protein controls. Research using these antibodies has expanded insights into B-cell tolerance, leukemia biomarkers, and potential therapeutic targets.
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