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产品描述：大鼠载脂蛋白H(Apo-H)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOH含量。APOH（载脂蛋白H）是一种参与脂质代谢的关键蛋白，在调节凝血和炎症方面发挥着重要作用。研究表明，APOH与多种疾病有关，包括自身免疫性疾病和心血管疾病。其研究机制主要涉及APOH与带负电物质（如肝素和磷脂）的相互作用，以及其在防止内在凝血级联激活中的作用。试剂盒检测范围为28 ng/mL-1800 ng/mL，适用于大鼠模型中Apo-H的定量分析，可支持脂代谢研究、心血管疾病机制探索以及免疫相关实验的开展，特别适用于基础科研中涉及脂蛋白调控、血栓形成或免疫应答机制的相关课题。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ApohBeta-2-glycoprotein 1 ELISA kit; Apolipoprotein H ELISA kit; Apo-H ELISA kit; Beta-2-glycoprotein I ELISA kit; B2GPI ELISA kit; Beta(2)GPI ELISA kit
缩写：APOH
Uniprot No.：P26644
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：28 ng/mL-1800 ng/mL
灵敏度：7 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:400 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:800 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:1600 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of rat Apo-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 107 | 101-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected18002.6552.5322.5942.4649002.1642.1172.1412.0114501.5971.5441.5711.4412250.8450.8280.8370.7071120.5010.5110.5060.376560.3070.3000.3040.174280.2340.2220.2280.09800.1310.1290.130 | ||||||
货期：3-5 working days
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