首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠B因子(BF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的BF含量。试剂盒检测范围为0.312 μg/mL-20 μg/mL，科研人员可将其应用于小鼠感染模型、自身免疫疾病造模、药物干预实验等场景中，定量分析血清或血浆BF水平变化，为探索补体系统在先天免疫中的作用、炎症反应调控机制或免疫调节剂开发提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cfb ELISA kit; Bf ELISA kit; H2-BfComplement factor B ELISA kit; EC 3.4.21.47 ELISA kit; C3/C5 convertase) [Cleaved into: Complement factor B Ba fragment; Complement factor B Bb fragment] ELISA kit
缩写：BF
Uniprot No.：P04186
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：0.312 μg/mL-20 μg/mL
灵敏度：0.078 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 92-104 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:800 | Average % | 102 | ||||
| Range % | 96-108 | |||||
回收率：
| The recovery of mouse BF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 82-94 | ||||
| EDTA plasma (n=4) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| µg/mlOD1OD2AverageCorrected 202.7122.8112.7622.608 102.1122.2122.1622.008 51.4281.4471.4381.284 2.50.8570.8660.8620.708 1.250.5240.5120.5180.364 0.6250.3500.3410.3460.192 0.3120.2680.2630.2660.112 00.1540.1530.154 | ||||||
货期：3-5 working days
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