Rabbit Polyclonal CHCHD5 Antibody

The CHCHD5 antibody is a crucial tool for investigating the CHCHD5 protein, a member of the coiled-coil-helix-coiled-coil-helix (CHCHD) domain-containing protein family. CHCHD5 is primarily localized to mitochondria and plays roles in maintaining mitochondrial structure, regulating oxidative phosphorylation, and modulating apoptosis. It is implicated in various physiological processes, including cellular energy metabolism and stress responses. Dysregulation of CHCHD5 has been linked to cancers, neurodegenerative diseases (e.g., Alzheimer’s and Parkinson’s), and cardiovascular disorders. Researchers utilize CHCHD5 antibodies in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study its expression patterns, subcellular localization, and interactions with mitochondrial proteins. These antibodies are typically validated using knockout cell lines or tissues to ensure specificity. Challenges include potential cross-reactivity with homologous family members (e.g., CHCHD2 or CHCHD10) and variability in detection due to post-translational modifications. Recent studies highlight its potential as a biomarker for cancer prognosis and therapeutic targeting. However, standardized protocols for antibody validation and application remain essential to ensure reproducibility across studies. Commercial CHCHD5 antibodies are available from multiple suppliers, often with species reactivity tailored to human, mouse, or rat samples.