| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | Mic19; MINOS3; PPP1R22 |
| WB Predicted band size | 26 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CHCHD3抗体的3篇参考文献示例(注：部分文献信息为虚构，仅作格式参考)：
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1. **文献名称**：*CHCHD3 maintains mitochondrial cristae structure and interacts with the MICOS complex*
**作者**：Anderson, C.J., et al.
**摘要**：本研究利用CHCHD3特异性抗体(Western blot/IF)揭示了CHCHD3在线粒体嵴形态维持中的关键作用，并证实其与MICOS复合体的相互作用，调控线粒体内膜结构。
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2. **文献名称**：*Loss of CHCHD3 impairs mitochondrial respiration and promotes Parkinson’s disease-related pathology*
**作者**：Smith, L., et al.
**摘要**：通过CHCHD3抗体检测人脑组织样本，发现帕金森病患者CHCHD3表达显著降低。功能实验表明，CHCHD3缺失导致线粒体呼吸链复合体活性受损，加剧神经元退化。
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3. **文献名称**：*Generation and validation of a polyclonal antibody targeting human CHCHD3 for mitochondrial studies*
**作者**：Zhang, Y., et al.
**摘要**：本文报道了一种针对人源CHCHD3蛋白的多克隆抗体的开发，通过免疫兔获得抗体，并经ELISA、免疫沉淀和免疫荧光验证其特异性，为线粒体蛋白研究提供新工具。
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(注：以上文献为示例，实际引用时请核实真实文献信息。)
CHCHD3 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 3) is a mitochondrial protein belonging to the CHCHD family, characterized by twin CHCH domains that mediate protein-protein interactions and subcellular localization. Primarily located in the mitochondrial intermembrane space, CHCHD3 plays a critical role in maintaining cristae structure, mitochondrial membrane integrity, and oxidative phosphorylation. It interacts with the MICOS (mitochondrial contact site and cristae organizing system) complex, which governs mitochondrial morphology and function. Dysregulation of CHCHD3 has been linked to mitochondrial disorders, neurodegenerative diseases (e.g., Parkinson’s and Alzheimer’s), and cancer due to its influence on apoptosis and metabolic reprogramming.
CHCHD3 antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess CHCHD3 levels in disease models or tissue samples. Specific antibodies help identify CHCHD3’s dual role in mitochondrial dynamics and nuclear signaling under stress conditions. Commercial antibodies are typically validated using knockout cell lines or siRNA silencing to ensure specificity. Research utilizing these antibodies has advanced understanding of mitochondrial pathophysiology, particularly in contexts of energy metabolism defects and age-related degeneration. However, challenges remain in standardizing antibody performance across experimental platforms, emphasizing the need for rigorous validation in diverse biological systems.
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