首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠双糖链蛋白聚糖(BGN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BGN含量。BGN作为靶点，其它在特定生理和病理过程中发挥作用。研究机制上，围绕其在细胞信号传导、分子相互作用等层面展开探究，旨在明确其功能及与疾病的关联，为相关疾病的诊断、治疗方案制定及新药研发提供依据。试剂盒检测范围为0.625 ng/mL-40 ng/mL，科研人员可通过该产品探究BGN在组织纤维化模型（如肝纤维化、肺纤维化）、肿瘤发生发展机制、炎症性疾病中的动态变化，或用于评估基因编辑动物模型中BGN的表达调控效果，为细胞外基质相关分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bgn ELISA Kit; Biglycan ELISA Kit; Bone/cartilage proteoglycan I ELISA Kit; PG-S1 ELISA Kit
缩写：BGN
Uniprot No.：P28653
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 102 | ||||
| Range % | 99-103 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 95-103 | |||||
回收率：
| The recovery of mouse BGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 100-108 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.3782.4962.4372.319201.8631.9651.9141.796101.4321.4671.4501.33251.0251.0901.0580.9402.50.6660.6440.6550.5371.250.4370.4700.4540.3360.6250.2690.2880.2790.16100.1160.1190.118 | ||||||
货期：3-5 working days
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