| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MURR1; C2orf5 |
| WB Predicted band size | 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human COMMD1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于COMMD1抗体的参考文献及其摘要概括：
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1. **文献名称**: *COMMD1. a novel protein involved in the proteosomal degradation of the NF-κB subunit*
**作者**: Maine GN, Burstein E
**摘要**: 该研究首次发现COMMD1通过促进NF-κB亚基的泛素化降解调控炎症反应。实验中利用抗COMMD1抗体进行免疫共沉淀(Co-IP)，证实COMMD1与泛素连接酶复合物的相互作用，揭示其在NF-κB信号通路中的关键作用。
2. **文献名称**: *COMMD1 deficiency results in hepatic copper overload and altered transcriptional responses*
**作者**: de Bie P, van de Sluis B, Burstein E
**摘要**: 研究通过构建COMMD1缺陷小鼠模型，发现其肝脏铜代谢异常。采用抗COMMD1抗体的Western blot和免疫组化技术，证明COMMD1在肝细胞中的特异性表达缺失导致铜转运蛋白ATP7B功能紊乱，阐明了COMMD1在铜稳态中的作用机制。
3. **文献名称**: *COMMD1 interacts with the COPI vesicle complex to regulate endosomal pH and copper uptake*
**作者**: Materia S, Cater MA, Klomp LWJ
**摘要**: 本研究揭示COMMD1通过调控内体pH值影响铜离子摄取。通过抗COMMD1抗体的免疫荧光共定位实验，发现COMMD1与COPI囊泡复合物共定位，并证明其功能缺失导致内体酸化障碍及细胞内铜蓄积。
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这些文献均以COMMD1抗体为关键实验工具，聚焦于其在铜代谢、炎症调控及细胞内运输中的功能机制。如需更多文献或具体细节，可进一步检索PubMed或Web of Science数据库。
COMMD1 (COpper Metabolism MURR1 Domain-containing protein 1) is a member of the COMMD protein family, which comprises 10 members sharing a conserved C-terminal COMM domain. Initially identified for its role in copper homeostasis, COMMD1 interacts with ATP7B, a copper-transporting ATPase mutated in Wilson’s disease, and regulates copper excretion. Defects in COMMD1 are linked to copper toxicosis in canine models. Beyond copper metabolism, COMMD1 participates in diverse cellular processes, including NF-κB signaling modulation, ion transport, cell survival, and hypoxia responses. It suppresses NF-κB activity by promoting the degradation of its subunits, influencing inflammation and cancer progression. COMMD1 also interacts with proteins like XIAP and CCDC22. impacting copper transport, endosomal trafficking, and DNA damage responses.
COMMD1 antibodies are essential tools for studying its expression, localization, and interactions. They are used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to explore its roles in physiological and pathological contexts. Dysregulation of COMMD1 is implicated in diseases like Wilson’s disease, cancers, neurodegenerative disorders, and immune dysfunctions. Polyclonal antibodies detect multiple epitopes, offering high sensitivity but potential cross-reactivity, while monoclonal antibodies provide specificity for precise detection. These antibodies aid in elucidating COMMD1’s molecular mechanisms and its potential as a therapeutic target or biomarker in copper-related and inflammatory diseases.
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