| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CLN80; p80-coilin |
| WB Predicted band size | 63 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human COIL |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于COIL(coilin)抗体的3篇代表性文献，涵盖基础机制及疾病应用研究：
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1. **标题**：*"Coilin: The first 25 years"*
**作者**：Gall JG (2017)
**摘要**：
综述文章系统总结了coilin蛋白发现25年来的研究进展，重点讨论其作为Cajal体标志物的功能，以及在RNA剪接、端粒维持中的作用。文中强调了特异性抗体的开发如何推动Cajal体定位及coilin动态行为的研究，并提及抗体在疾病模型(如脊髓性肌萎缩)中的应用。
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2. **标题**：*"Autoantibodies to coilin in systemic lupus erythematosus"*
**作者**：Satoh M, et al. (2012)
**摘要**：
研究检测了系统性红斑狼疮(SLE)患者血清中抗coilin自身抗体的存在。通过免疫荧光和Western blot分析，发现约15%的SLE患者存在抗coilin抗体，且与特定临床症状相关，提示其可能作为疾病亚型的生物标志物。
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3. **标题**：*"Dynamic interaction between coilin and SMN complexes in Cajal bodies"*
**作者**：Hebert MD, et al. (2002)
**摘要**：
利用coilin抗体进行共免疫沉淀和共定位实验，揭示coilin与生存运动神经元(SMN)复合物在Cajal体中的动态相互作用，阐明了其在snRNP(小核核糖核蛋白)组装中的关键作用，为脊髓性肌萎缩的分子机制提供新见解。
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4. **标题**：*"CRISPR/Cas9-mediated knockout of coilin excludes its role in stress-induced transcription inhibition"*
**作者**：Sharma R, et al. (2020)
**摘要**：
通过CRISPR敲除结合coilin抗体验证，研究coilin在细胞应激反应中的功能。发现coilin缺失不影响应激诱导的转录暂停，但导致Cajal体结构解体，提示其结构功能独立于特定应激通路。
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**备注**：若需具体文献链接或补充其他研究领域(如病毒感染、癌症)，可进一步调整方向。
COIL antibody targets the protein COIL (Coatomer Interacting Lipid-binding protein), also known as coatomer-associated protein or coiled-coil domain-containing protein 80 (CCDC80). COIL is a conserved eukaryotic protein involved in intracellular vesicle trafficking and lipid metabolism, primarily interacting with components of the coatomer complex, which mediates retrograde transport from the Golgi apparatus to the endoplasmic reticulum. It plays roles in maintaining Golgi structure, regulating secretory pathways, and influencing cell adhesion and migration. COIL’s coiled-coil domains facilitate protein-protein interactions, critical for its function in membrane dynamics.
COIL antibodies are essential tools for studying these processes in molecular and cellular biology. They are used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect COIL expression, localization, and interaction partners. Research has linked COIL dysregulation to diseases, including cancer, where altered vesicle trafficking may promote tumor progression, and metabolic disorders tied to lipid processing defects. In neuroscience, COIL’s role in neuronal Golgi function and synaptic vesicle transport is of interest.
The development of COIL antibodies has enabled insights into its physiological and pathological mechanisms, though its full functional scope remains under investigation. These antibodies are valuable for both basic research and potential diagnostic applications, highlighting COIL’s emerging significance in cellular homeostasis and disease.
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