Rabbit Polyclonal DENND1B Antibody

The DENND1B antibody is a tool used to detect and study DENND1B (Differentially Expressed in Normal and Neoplastic Cells Domain 1B), a protein belonging to the DENN domain-containing family. DENND1B functions as a guanine nucleotide exchange factor (GEF) for Rab35 GTPase, regulating intracellular trafficking, membrane dynamics, and signaling pathways. It plays roles in cytokine secretion, receptor recycling, and immune cell activation. DENND1B is implicated in various diseases, including asthma, autoimmune disorders, and cancers, with genetic studies linking its variants to susceptibility in these conditions. The antibody enables researchers to investigate DENND1B's expression, localization, and interactions in cells or tissues through techniques like Western blotting, immunohistochemistry, and immunoprecipitation. Available in monoclonal or polyclonal forms (often raised in rabbits or mice), it is validated for specificity and reproducibility in diverse experimental models. Research using DENND1B antibodies has advanced understanding of its role in Rab35-mediated processes, such as endosomal sorting and T-cell receptor signaling, offering insights into disease mechanisms and potential therapeutic targets. Its utility spans basic cell biology and translational studies, particularly in immune dysregulation and oncology.