| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | XIB; G4.8; DNL1L; DNASEX; DNAS1L1 |
| WB Predicted band size | 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human DNASE1L1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及DNASE1L1抗体的参考文献及其摘要概括：
1. **文献名称**: *DNASE1L1 regulates innate immunity by suppressing interferon signaling*
**作者**: Kawane K. et al.
**摘要**: 研究利用特异性抗体验证DNASE1L1在巨噬细胞中的定位，发现其通过降解胞外DNA抑制cGAS-STING通路，从而调控I型干扰素产生(Nature Immunology, 2019)。
2. **文献名称**: *Deoxyribonuclease 1-like 1 is required for mitochondrial DNA clearance in apoptotic cells*
**作者**: Enari M. et al.
**摘要**: 通过Western blot和免疫荧光技术，证明DNASE1L1抗体标记的蛋白在凋亡过程中选择性切割线粒体DNA，防止其释放引发炎症(Cell Death & Differentiation, 2016)。
3. **文献名称**: *Antibody-based detection of DNASE1L1 in hepatocellular carcinoma*
**作者**: Li Y. et al.
**摘要**: 开发高特异性单克隆抗体，发现DNASE1L1在肝癌组织中表达显著下调，提示其可能作为肿瘤抑制因子(Journal of Clinical Pathology, 2020)。
注：以上为模拟示例，实际文献需通过PubMed/Google Scholar检索确认。
The DNASE1L1 (Deoxyribonuclease 1 Like 1) antibody is a tool used to detect and study the DNASE1L1 protein, a member of the DNase I family. DNASE1L1. also known as DNase γ, is a calcium-magnesium-dependent endonuclease implicated in DNA fragmentation during apoptosis. Unlike the secreted DNase I, DNASE1L1 localizes to the cytoplasm and mitochondria, playing roles in nuclear DNA degradation, mitochondrial genome maintenance, and innate immune responses by clearing cell-free DNA. Its dysregulation has been linked to autoimmune diseases, cancer progression, and impaired apoptotic processes.
Antibodies targeting DNASE1L1 are typically produced in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. These antibodies (polyclonal or monoclonal) enable researchers to investigate DNASE1L1 expression, localization, and function via techniques such as Western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. Validated antibodies are essential for distinguishing DNASE1L1 from homologous DNases (e.g., DNASE1. DNASE2) and confirming specificity through knockout controls. Applications span apoptosis studies, cancer biology (e.g., tumor suppression roles), and autoimmune disorder research (e.g., systemic lupus erythematosus linked to defective DNA clearance). Recent studies also explore its potential in mitochondrial DNA release-related inflammation (e.g., in sepsis or neurodegenerative diseases). Reliable DNASE1L1 antibodies thus support mechanistic insights into cell death, genome stability, and therapeutic target discovery.
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