| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | L1MT; BM022; MRP-L1 |
| WB Predicted band size | 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human MRPL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于MRPL1抗体的模拟参考文献示例(实际文献需通过学术数据库检索)：
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1. **文献名称**：*MRPL1 Antibody Characterization in Mitochondrial Ribosome Assembly Studies*
**作者**：Chen L, et al.
**摘要**：本研究验证了兔源多克隆MRPL1抗体的特异性，通过Western blot和免疫荧光技术证实其在哺乳动物细胞线粒体中的定位。研究发现MRPL1缺失导致线粒体核糖体大亚基组装异常，提示其在氧化磷酸化相关疾病中的潜在作用。
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2. **文献名称**：*MRPL1 as a Novel Biomarker in Colorectal Cancer: An Immunohistochemical Approach*
**作者**：Kim S, et al.
**摘要**：利用商业化MRPL1抗体对结直肠癌组织进行免疫组化分析，发现MRPL1表达水平与肿瘤代谢活跃程度正相关。该抗体可作为评估线粒体功能失调和癌症进展的生物标志物。
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3. **文献名称**：*Development of a Monoclonal Antibody Targeting MRPL1 for Neurodegenerative Disease Models*
**作者**：Gomez-Ramos P, et al.
**摘要**：报道了一种新型小鼠单克隆MRPL1抗体的开发，用于检测阿尔茨海默病模型中线粒体核糖体蛋白表达变化。实验表明，MRPL1下调与神经元能量代谢障碍显著相关。
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**建议**：
可通过 **PubMed** 或 **Google Scholar** 搜索真实文献，关键词：
`MRPL1 antibody mitochondrial ribosome`、`MRPL1 western blot validation`、`MRPL1 disease biomarker`。
注意筛选近5年文献以获取最新进展。
The mitochondrial ribosomal protein L1 (MRPL1) is a component of the large subunit of the mitochondrial ribosome, essential for mitochondrial protein synthesis. Mitochondria possess their own ribosomes (mitoribosomes) that translate mitochondrial DNA-encoded mRNAs, producing subunits of the oxidative phosphorylation (OXPHOS) complexes critical for ATP production. MRPL1. encoded by nuclear DNA, is imported into mitochondria and plays a structural or functional role in maintaining mitoribosome integrity and facilitating translation.
MRPL1-specific antibodies are vital tools for studying mitochondrial gene expression, ribosome biogenesis, and respiratory chain defects. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess MRPL1 expression levels, subcellular localization, and interactions. Dysregulation of MRPL1 has been linked to mitochondrial disorders, cancer, and neurodegenerative diseases, making these antibodies valuable in both basic and clinical research. For instance, reduced MRPL1 levels may indicate impaired mitochondrial translation, aiding in the diagnosis of metabolic syndromes.
When selecting MRPL1 antibodies, researchers prioritize specificity, validated applications, and cross-reactivity with relevant species (e.g., human, mouse). Many commercial antibodies are raised against peptide sequences unique to MRPL1 and undergo rigorous validation using knockout controls or siRNA-mediated silencing. Proper controls, such as mitochondrial markers, are recommended to confirm results. These tools continue to advance our understanding of mitochondrial dysfunction and its role in disease pathogenesis.
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