| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ATPBD1B |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GPN2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GPN2抗体的3篇文献示例(内容为模拟概括，具体文献需根据实际研究补充)：
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1. **文献名称**：*GPN2 regulates the localization and assembly of RNA polymerase II*
**作者**：Smith A, et al.
**摘要**：本研究利用GPN2特异性抗体，通过免疫荧光和Western blot技术，揭示了GPN2蛋白在RNA聚合酶II(RNAPII)的核质运输和复合体组装中的关键作用。实验表明，GPN2缺失会导致RNAPII滞留于细胞质，影响转录活性。
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2. **文献名称**：*GPN2 interacts with RPB1 and modulates its stability in human cells*
**作者**：Chen L, et al.
**摘要**：通过免疫共沉淀(Co-IP)和GPN2抗体的应用，研究发现GPN2与RNA聚合酶II最大亚基RPB1直接结合。敲低GPN2会导致RPB1降解，提示其在维持RNAPII稳定性中的功能，可能参与癌症相关通路。
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3. **文献名称**：*Functional characterization of GPN-loop GTPases in cell cycle progression*
**作者**：Wang Y, et al.
**摘要**：该研究利用GPN2抗体进行细胞周期同步化实验，发现GPN2在S期和G2/M期表达上调。通过抗体标记，证实GPN2在细胞核内的动态定位变化与DNA复制及有丝分裂进程相关。
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如需具体文献，建议通过PubMed或Google Scholar以“GPN2 antibody”或“GPN2 function”为关键词检索近期研究。
The GPN2 antibody is a research tool designed to target the GPN-loop GTPase 2 protein, a conserved eukaryotic enzyme encoded by the *GPN2* gene. GPN2 belongs to the GPN (GLYCOPIN) family of GTPases, which play critical roles in RNA polymerase II (RNAP II) assembly, nuclear transport, and DNA repair. Structurally, GPN2 contains a distinctive glycine-proline-asparagine (GPN) motif, essential for its GTPase activity and interaction with RNAP II subunits. Studies suggest that GPN2 collaborates with GPN1 and GPN3 to form a trimeric complex that stabilizes RNAP II during its biogenesis and nuclear localization. Dysregulation of GPN2 has been linked to transcriptional defects, genomic instability, and pathologies such as cancer, where its overexpression correlates with tumor progression.
The GPN2 antibody is widely used in molecular biology to investigate protein expression, localization, and function through techniques like Western blotting, immunofluorescence, and immunoprecipitation. It aids in elucidating mechanisms of RNAP II dynamics, cell cycle regulation, and stress responses. Commercial GPN2 antibodies are typically raised in rabbits or mice using synthetic peptides or recombinant proteins as immunogens, and their specificity is validated via knockout controls or siRNA-mediated knockdown. As RNAP II dysfunction is implicated in numerous diseases, this antibody remains vital for advancing studies in transcription biology, cancer research, and therapeutic target discovery.
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