| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | AC40; RPA5; TCS3; HLD11; RPA39; RPA40; RPAC1; RPC40 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human POLR1C |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于POLR1C抗体的示例参考文献(虚拟构建，供参考)：
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1. **文献名称**: *POLR1C mutations impair RNA polymerase I/III assembly and cause hypomyelinating leukodystrophy*
**作者**: Smith A, et al.
**摘要**: 本研究利用POLR1C抗体进行免疫沉淀和蛋白质组学分析，发现POLR1C突变导致RNA聚合酶I/III复合体组装缺陷，与婴儿脑白质病变相关，揭示了其在髓鞘形成中的关键作用。
2. **文献名称**: *Antibody-based profiling of POLR1C expression in Treacher Collins syndrome*
**作者**: Chen L, et al.
**摘要**: 通过POLR1C特异性抗体检测患者细胞，发现POLR1C蛋白表达水平降低与颅面发育异常(如Treacher Collins综合征)相关，提示其作为疾病生物标志物的潜力。
3. **文献名称**: *Development of a monoclonal antibody targeting POLR1C for functional studies in zebrafish models*
**作者**: Kim S, et al.
**摘要**: 报道一种新型POLR1C单克隆抗体的开发，并应用于斑马鱼模型，证实POLR1C在胚胎发育中的必要性，为研究RNA聚合酶III功能障碍提供了工具。
4. **文献名称**: *POLR1C interacts with SMN protein in motor neurons: Evidence from co-immunoprecipitation assays*
**作者**: Gonzalez M, et al.
**摘要**: 使用POLR1C抗体进行共免疫沉淀实验，发现其与脊髓性肌萎缩症(SMA)相关蛋白SMN存在相互作用，暗示POLR1C在神经元存活中的新机制。
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**注意**：以上文献为示例，实际文献请通过PubMed、Google Scholar等数据库检索关键词“POLR1C antibody”或“POLR1C function”获取。
The POLR1C antibody targets a subunit of RNA polymerase I and III, essential enzymes responsible for synthesizing ribosomal RNA (rRNA) and small RNAs, including tRNA and 5S rRNA. POLR1C (RNA Polymerase I and III Subunit C) is a shared component of both polymerase complexes, playing a critical role in ribosomal biogenesis and transcriptional regulation. Mutations in the POLR1C gene are linked to autosomal recessive disorders such as Treacher Collins syndrome (TCS) and hypomyelinating leukodystrophy with hypodontia (HVDRR), characterized by craniofacial abnormalities or neurological deficits.
POLR1C antibodies are widely used in research to study RNA polymerase assembly, subcellular localization, and functional interactions. They aid in elucidating molecular mechanisms underlying POLR1C-associated diseases, including dysregulation of ribosome synthesis and cellular stress responses. These antibodies are applied in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect POLR1C expression in tissues or cultured cells. Commercial POLR1C antibodies are typically validated for specificity using knockout controls or siRNA knockdown. Researchers also utilize them to explore therapeutic strategies, such as gene therapy or small-molecule interventions, for POLR1C-related disorders. As a tool, POLR1C antibodies bridge genetic findings to functional insights in developmental biology and rare disease research.
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