| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human TCN2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及TCN2抗体的研究文献示例(信息经过概括整理，非真实文献)：
1. **《Transcobalamin II deficiency: characterization of two novel mutations and functional analysis of mutant alleles》**
- 作者：Rosenblatt DS et al.
- 摘要：研究通过开发特异性TCN2抗体，分析了两种新型TCN2基因突变导致维生素B12代谢缺陷的分子机制，揭示了突变对蛋白质稳定性和功能的影响。
2. **《TCN2 as a potential biomarker for colorectal cancer progression》**
- 作者：Wang L et al.
- 摘要：利用TCN2抗体进行免疫组化分析，发现结直肠癌组织中TCN2表达水平与肿瘤分期和患者预后显著相关，提示其可能作为癌症进展的生物标志物。
3. **《Autoantibodies against transcobalamin II in autoimmune vitamin B12 malabsorption》**
- 作者：Quadros EV et al.
- 摘要：通过ELISA和Western blot验证了自身免疫性维生素B12吸收障碍患者血清中存在抗TCN2抗体，并证明其干扰维生素B12的转运功能。
4. **《Neuroprotective role of TCN2 in Alzheimer's disease: Insights from mouse models》**
- 作者：Miller JW et al.
- 摘要：采用TCN2抗体检测阿尔茨海默病模型小鼠脑组织中TCN2的分布变化，发现其表达降低与神经退行性病变及维生素B12脑内运输受损相关。
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注：以上为模拟文献，实际研究中建议通过PubMed或Web of Science以关键词“TCN2 antibody”检索真实论文。如需具体文献，可进一步提供研究方向(如疾病、技术方法等)。
TCN2 (Transcobalamin II) is a key plasma protein responsible for the transport of vitamin B12 (cobalamin) into cells. It binds to dietary vitamin B12 in the intestines and facilitates its absorption into the bloodstream, after which the TCN2-B12 complex is internalized by cells via receptor-mediated endocytosis. This process is critical for cellular functions, including DNA synthesis, red blood cell formation, and neurological health.
Antibodies targeting TCN2 are essential tools for studying its expression, distribution, and role in vitamin B12 metabolism. They are widely used in immunoassays (e.g., ELISA, Western blot) to quantify TCN2 levels in serum or tissues, aiding in the diagnosis of conditions like vitamin B12 deficiency, congenital TCN2 defects, or autoimmune disorders where anti-TCN2 autoantibodies may impair vitamin uptake. Research applications also include investigating TCN2's involvement in cancer progression, as some tumors overexpress TCN2 to meet high metabolic demands.
Commercial TCN2 antibodies are typically raised in hosts like rabbits or mice, with validation in specificity and cross-reactivity across species (human, mouse, rat). Their development has advanced understanding of cobalamin-related pathologies and therapeutic strategies, such as targeted drug delivery systems leveraging TCN2 receptors.
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