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Human C4 binding protein,C4BP ELISA Kit

  • 中文名: 人C4结合蛋白(C4BP)ELISA Kit
货号: IPD-E11170h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人C4结合蛋白(C4BP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆样本中的C4BPA含量。C4BPA作为一个靶点,其它在补体系统等生理过程中发挥作用。在研究机制上,科研人员聚焦于其在疾病发生发展中的信号通路、与其他分子的相互作用,以此探索它作为治疗靶点的可能性,为相关疾病药物研发提供方向。试剂盒检测范围为39 ng/ml- 2500 ng/ml,适用于基础医学研究中对补体系统功能分析、疾病机制探索及生物标志物筛选等科研场景,为探索免疫调控网络或药物干预效果提供可靠工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    C4b-binding protein alpha chain ELISA Kit; C4bp ELISA Kit; C4bpa ELISA Kit; C4BPA_HUMAN ELISA Kit; Complement component 4 binding protein alpha ELISA Kit; Complement component 4 binding protein ELISA Kit; Proline-rich protein ELISA Kit; PrP ELISA Kit; RP11-164O23.4 ELISA Kit
  • 缩写:
    C4BPA
  • Uniprot No.:
    P04003
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma
  • 检测范围:
    39 ng/ml - 2500 ng/ml
  • 灵敏度:
    9.75 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human C4BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:100

    Average %

    92


    Range %

    87-96


    1:200

    Average %

    95


    Range %

    90-99


    1:400

    Average %

    90


    Range %

    85-96


    1:800

    Average %

    93


    Range %

    89-98


  • 回收率:

    The recovery of human C4BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    98

    94-103


    EDTA plasma (n=4)

    89

    82-95


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    2500

    2.931

    2.812

    2.872

    2.736


    1250

    2.457

    2.349

    2.403

    2.267


    625

    1.678

    1.628

    1.653

    1.517


    312.5

    0.860

    0.816

    0.838

    0.702


    156.25

    0.490

    0.467

    0.479

    0.343


    78

    0.321

    0.317

    0.319

    0.183


    39

    0.199

    0.189

    0.194

    0.058


    0

    0.141

    0.131

    0.136




  • 货期:
    3-5 working days

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