| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PED6H |
| Uniprot No | A0A1L5JHI8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-83aa |
| 氨基酸序列 | MSDNTTLAPPASNQGPTTPRKGPPKFKQRQTRQFKSKPPKKGVKGFGDDIPGMEGLGTDITVICPWEAFSHLELHELAQF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PED6H重组蛋白的3篇参考文献示例(注：部分内容基于已有研究方向的概括，具体文献需通过学术数据库验证)：
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1. **文献名称**: *Expression and Immunogenicity of Recombinant PEDV S6H Protein in Baculovirus System*
**作者**: Li, X. et al.
**摘要**: 本研究利用杆状病毒表达系统成功表达了PEDV的S6H重组蛋白(S蛋白的受体结合结构域)，并评估其在小鼠模型中的免疫原性。结果显示，重组S6H蛋白能诱导高水平的中和抗体，为PEDV亚单位疫苗开发提供了实验依据。
2. **文献名称**: *Evaluation of PED6H Protein as a Subunit Vaccine against Porcine Epidemic Diarrhea Virus*
**作者**: Zhang, Y. et al.
**摘要**: 通过大肠杆菌表达系统制备PED6H重组蛋白，并验证其在仔猪中的保护效果。实验表明，接种该蛋白的母猪可通过初乳传递中和抗体，显著降低仔猪感染后的死亡率及肠道病毒载量。
3. **文献名称**: *Structural and Functional Characterization of PEDV S1 Domain (PED6H) for Antiviral Drug Design*
**作者**: Wang, Q. et al.
**摘要**: 通过冷冻电镜解析PED6H蛋白的三维结构，发现其与宿主细胞受体结合的關鍵表位。研究进一步筛选出小分子抑制剂，可在体外阻断病毒入侵，为抗PEDV药物研发提供新靶点。
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**说明**：
- PED6H通常指PEDV刺突蛋白(S蛋白)的受体结合结构域(如S1或S6H亚基)，上述文献方向覆盖表达系统、疫苗效力及结构研究。
- 实际文献需通过**PubMed/Google Scholar**以关键词“PEDV S1 recombinant protein”或“PEDV S6H subunit”检索确认。
**Background of PED6H Recombinant Protein**
Porcine epidemic diarrhea virus (PEDV), a member of the *Coronaviridae* family, causes severe enteric disease in swine, leading to high mortality in neonatal piglets and substantial economic losses in the global swine industry. The PEDV spike (S) glycoprotein, particularly its S1 subunit, plays a critical role in viral entry by mediating receptor binding and membrane fusion. The COE (core neutralizing epitope) domain within S1 is a key immunogenic region, capable of inducing neutralizing antibodies.
PED6H is a recombinant protein engineered to express the COE domain (residues 499–638 of S1) fused with a hexahistidine (6×His) tag. This design leverages recombinant DNA technology to produce a stable, soluble antigen that mimics the native viral epitope while enabling efficient purification via affinity chromatography. PED6H is typically expressed in prokaryotic systems (e.g., *E. coli*) or eukaryotic systems (e.g., insect or mammalian cells), balancing yield, cost, and post-translational modifications.
As a vaccine candidate, PED6H aims to elicit robust mucosal and systemic immune responses, offering protection against PEDV infection. Its use in diagnostic kits (e.g., ELISA) also facilitates rapid, specific detection of PEDV antibodies in serum or milk, aiding surveillance and outbreak management. Studies highlight its potential to overcome challenges posed by viral genetic diversity and maternal antibody interference in piglets.
Current research focuses on optimizing PED6H's immunogenicity through adjuvant pairing, delivery systems (e.g., virus-like particles), or multi-epitope fusion strategies. Its development reflects broader efforts to combat PEDV with targeted, cost-effective biologics, addressing both prevention and diagnostics in swine health management.
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