| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PELI2 |
| Uniprot No | Q9HAT8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-420aa |
| 氨基酸序列 | MFSPGQEEHC APNKEPVKYG ELVVLGYNGA LPNGDRGRRK SRFALYKRPK ANGVKPSTVH VISTPQASKA ISCKGQHSIS YTLSRNQTVV VEYTHDKDTD MFQVGRSTES PIDFVVTDTI SGSQNTDEAQ ITQSTISRFA CRIVCDRNEP YTARIFAAGF DSSKNIFLGE KAAKWKNPDG HMDGLTTNGV LVMHPRGGFT EESQPGVWRE ISVCGDVYTL RETRSAQQRG KLVESETNVL QDGSLIDLCG ATLLWRTADG LFHTPTQKHI EALRQEINAA RPQCPVGLNT LAFPSINRKE VVEEKQPWAY LSCGHVHGYH NWGHRSDTEA NERECPMCRT VGPYVPLWLG CEAGFYVDAG PPTHAFTPCG HVCSEKSAKY WSQIPLPHGT HAFHAACPFC ATQLVGEQNC IKLIFQGPID |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PELI2重组蛋白的示例性参考文献(内容为虚构，仅作参考)：
1. **文献名称**：*Structural and functional characterization of recombinant PELI2 protein in TLR signaling*
**作者**：Li, X., et al.
**摘要**：本研究成功表达并纯化了重组人源PELI2蛋白，通过体外实验证实其作为E3泛素连接酶参与TLR/IL-1R信号通路，调控IRAK1泛素化修饰，影响NF-κB激活。
2. **文献名称**：*PELI2 recombinant protein suppresses tumor growth by modulating macrophage polarization*
**作者**：Wang, Y., et al.
**摘要**：通过大肠杆菌系统表达PELI2重组蛋白，发现其通过促进M1型巨噬细胞分化抑制肿瘤微环境中的炎症反应，在黑色素瘤小鼠模型中显著延缓肿瘤进展。
3. **文献名称**：*Crystal structure of the PELI2 ubiquitin ligase domain and its interaction with UBC13*
**作者**：Zhang, H., et al.
**摘要**：解析了重组PELI2蛋白的泛素连接酶结构域晶体结构，揭示了其与泛素结合酶UBC13的互作界面，为设计靶向PELI2的小分子抑制剂提供结构基础。
4. **文献名称**：*PELI2 knockout and recombinant protein rescue experiments reveal its role in autoimmune encephalomyelitis*
**作者**：Kim, J., et al.
**摘要**：利用重组PELI2蛋白回补实验，证明其在多发性硬化症模型中通过抑制Th17细胞分化减轻神经炎症，提示其作为潜在治疗靶点。
**注意**：以上文献为模拟内容，实际研究中请通过PubMed/Google Scholar等数据库检索真实文献。如需具体文献支持，可补充关键词进一步筛选。
**Background of PELI2 Recombinant Protein**
PELI2 (Pellino E3 Ubiquitin Protein Ligase Family Member 2) is a member of the Pellino family of E3 ubiquitin ligases, which play critical roles in regulating immune and inflammatory signaling pathways. Initially identified as adaptor proteins in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling, Pellino proteins, including PELI2. mediate ubiquitination events that modulate downstream signaling cascades, particularly those involving nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. PELI2 is characterized by a conserved N-terminal forkhead-associated (FHA) domain, which facilitates phosphothreonine-dependent protein interactions, and a C-terminal RING-like domain responsible for its E3 ligase activity.
Recombinant PELI2 protein is engineered using expression systems (e.g., *E. coli*, mammalian, or insect cells*) to produce purified, functional PELI2 for biochemical and cellular studies. Its recombinant form enables researchers to dissect its role in ubiquitinating substrates like IRAK1 (Interleukin-1 Receptor-Associated Kinase 1) or TRAF6 (TNF Receptor-Associated Factor 6), thereby regulating innate immune responses, cell survival, and apoptosis. Dysregulation of PELI2 has been implicated in inflammatory diseases, autoimmune disorders, and cancer, highlighting its therapeutic potential.
Studies using recombinant PELI2 have also explored its non-canonical functions, such as modulating neuronal signaling and metabolic pathways. The protein’s dual role as a scaffold and enzyme underscores its complexity in cellular homeostasis. By leveraging recombinant PELI2. researchers aim to develop targeted therapies or diagnostic tools for diseases linked to aberrant ubiquitination or immune signaling.
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