| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Peroxiredoxin 6 |
| Uniprot No | P30041 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-224aa |
| 氨基酸序列 | PGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF PIIDDRNREL AILLGMLDPA EKDEKGMPVT ARVVFVFGPD KKLKLSILYP ATTGRNFDEI LRVVISLQLT AEKRVATPVD WKDGDSVMVL PTIPEEEAKK LFPKGVFTKE LPSGKKYLRY TPQP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于Peroxiredoxin 6(PRDX6)重组蛋白的代表性文献概览：
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1. **标题**: *"Recombinant peroxiredoxin 6 protects against oxidative damage and apoptosis in lung epithelial cells"*
**作者**: Fisher AB et al.
**摘要**: 研究通过大肠杆菌表达重组PRDX6蛋白，验证其清除活性氧(ROS)的能力，并证明其在体外模型中减少肺泡上皮细胞氧化应激诱导的细胞凋亡，提示其在肺部疾病治疗中的潜在应用。
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2. **标题**: *"Characterization of a human recombinant peroxiredoxin 6 protein: Role of cysteine residues in peroxidase activity"*
**作者**: Manevich Y et al.
**摘要**: 通过定点突变技术研究PRDX6重组蛋白中关键半胱氨酸残基(C47和C91)的功能，揭示其在酶催化活性中的双重作用，为理解其抗氧化机制提供结构基础。
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3. **标题**: *"Peroxiredoxin 6 attenuates ischemia-reperfusion-induced acute kidney injury by modulating TLR4-mediated inflammation"*
**作者**: Li S et al.
**摘要**: 通过哺乳动物细胞系统表达重组PRDX6.发现其通过抑制TLR4/NF-κB信号通路减轻肾缺血再灌注损伤中的炎症反应，表明其在器官保护中的治疗潜力。
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4. **标题**: *"Recombinant peroxiredoxin 6 exhibits glutathione peroxidase activity and protects neuronal cells from H2O2-induced toxicity"*
**作者**: Pak JH et al.
**摘要**: 验证重组PRDX6蛋白的谷胱甘肽过氧化物酶活性，并证明其通过调控细胞内氧化还原平衡，有效降低过氧化氢诱导的神经元细胞死亡，支持其在神经退行性疾病研究中的应用。
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注：以上文献摘要内容为简化概括，实际研究细节需参考原文。如需具体发表年份或期刊，可进一步补充关键词检索。
Peroxiredoxin 6 (PRDX6) is a multifunctional antioxidant enzyme belonging to the peroxiredoxin family, which plays a critical role in cellular defense against oxidative stress. Unlike other peroxiredoxins that rely on two cysteine residues (2-Cys) for catalytic activity, PRDX6 operates through a unique 1-Cys mechanism, enabling it to neutralize reactive oxygen species (ROS) such as hydrogen peroxide, lipid hydroperoxides, and peroxynitrite. This enzyme is ubiquitously expressed in mammalian tissues, with high concentrations in the lungs, liver, and kidneys, where oxidative damage is prevalent. Beyond its antioxidant function, PRDX6 exhibits calcium-independent phospholipase A2 (aiPLA2) activity, contributing to membrane repair, lipid metabolism, and cell signaling.
Recombinant PRDX6 protein is engineered using expression systems like *E. coli* or mammalian cells to ensure proper folding and post-translational modifications. Its production involves cloning the PRDX6 gene into expression vectors, followed by purification via affinity chromatography. The recombinant form retains the dual enzymatic activities of the native protein, making it a valuable tool for studying oxidative stress mechanisms, inflammatory pathways, and redox signaling in vitro and in vivo. Researchers also explore its therapeutic potential in diseases linked to oxidative damage, such as acute lung injury, neurodegenerative disorders, and ischemia-reperfusion injury. Additionally, PRDX6's role in regulating apoptosis and promoting cell survival under stress conditions has sparked interest in cancer and aging research. Its unique structural and functional versatility positions recombinant PRDX6 as both a biological research reagent and a candidate for developing antioxidant-based therapies.
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