| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTX3 |
| Uniprot No | P26022 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-381aa |
| 氨基酸序列 | ENSDDYDLMYVNLDNEIDNGLHPTEDPTPCACGQEHSEWDKLFIMLENSQ MRERMLLQATDDVLRGELQRLREELGRLAESLARPCAPGAPAEARLTSAL DELLQATRDAGRRLARMEGAEAQRPEEAGRALAAVLEELRQTRADLHAVQ GWAARSWLPAGCETAILFPMRSKKIFGSVHPVRPMRLESFSACIWVKATD VLNKTILFSYGTKRNPYEIQLYLSYQSIVFVVGGEENKLVAEAMVSLGRW THLCGTWNSEEGLTSLWVNGELAATTVEMATGHIVPEGGILQIGQEKNGC CVGGGFDETLAFSGRLTGFNIWDSVLSNEEIRETGGAESCHIRGNIVGWG VTEIQPHGGAQYVS |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTX3重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*PTX3 as a paradigm of the long pentraxin family: A component of the humoral arm of innate immunity*
**作者**：Bottazzi B, et al.
**摘要**：该综述总结了PTX3在先天免疫中的作用，强调其作为模式识别分子的功能。研究提到重组PTX3蛋白的生产及其在炎症和感染模型中的应用，证实其通过结合病原体相关分子模式(PAMPs)增强宿主防御。
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2. **文献名称**：*Non-redundant role of the long pentraxin PTX3 in anti-fungal innate immune response*
**作者**：Garlanda C, et al.
**摘要**：通过基因敲除小鼠模型，研究发现PTX3缺陷导致对烟曲霉菌易感性增加。重组PTX3蛋白的补充可恢复抗真菌免疫，表明其在调理病原体、促进吞噬作用中的关键作用。
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3. **文献名称**：*Therapeutic potential of recombinant PTX3 in fungal keratitis: Suppression of inflammation and fungal clearance*
**作者**：Jeon H, et al.
**摘要**：该研究利用重组PTX3蛋白治疗真菌性角膜炎小鼠模型，证明其通过抑制过度炎症反应(如TNF-α和IL-6)并直接结合真菌表面，增强中性粒细胞的杀菌活性，显著改善疾病预后。
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**注**：以上文献信息基于领域内代表性研究整理，具体发表年份和期刊可能需要通过数据库(如PubMed)进一步核实。
**Background of Recombinant PTX3 Protein**
Pentraxin 3 (PTX3), a member of the long pentraxin family, is a multifunctional glycoprotein involved in innate immunity, inflammation, and tissue remodeling. Unlike its short counterpart C-reactive protein (CRP), PTX3 is produced locally by various cells—including endothelial cells, macrophages, dendritic cells, and fibroblasts—in response to proinflammatory cytokines (e.g., TNF-α, IL-1β) or pathogen-associated molecular patterns (PAMPs). Structurally, PTX3 contains an N-terminal domain unique to long pentraxins and a C-terminal pentraxin domain, enabling interactions with pathogens, complement components, and extracellular matrix proteins.
Recombinant PTX3 refers to the protein produced via genetic engineering, typically using mammalian expression systems (e.g., CHO cells) or bacterial platforms, ensuring high purity and scalability. Its recombinant form retains the functional properties of native PTX3. such as binding to microbial moieties (e.g., fungal polysaccharides, bacterial components) and modulating immune responses. PTX3 acts as a soluble pattern recognition receptor (PRR), opsonizing pathogens for phagocytosis, regulating complement activation, and fine-tuning inflammation to prevent tissue damage.
Research highlights PTX3's dual role: it promotes pathogen clearance while dampening excessive inflammation, making it a potential therapeutic target in sepsis, COVID-19. and autoimmune diseases. Additionally, PTX3 is implicated in tissue repair, cancer progression, and female fertility, reflecting its pleiotropic functions. Recombinant PTX3 is widely used in preclinical studies to explore its diagnostic and therapeutic potential, including as a biomarker for infectious or inflammatory conditions or as an immunomodulatory agent. Its ability to interact with FGF2 and regulate angiogenesis further underscores its relevance in regenerative medicine. Overall, recombinant PTX3 serves as a critical tool for dissecting its biological roles and translational applications.
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