| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PEPD |
| Uniprot No | P12955 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-493aa |
| 氨基酸序列 | AAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGG EETQRYCTDTGVLFRQESFFHWAFGVTEPGCYGVIDVDTGKSTLFVPRLP ASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQKPSVLLTLRGVN TDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKI SSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENS AVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTAD QKAVYEAVLRSSRAVMGAMKPGVWW PDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDV HDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEAL ADPARASFFNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIE ACMAGCDKAFTPFSGPK |
| 预测分子量 | 54 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PEPD重组蛋白的代表性文献摘要概述：
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1. **标题**：Expression and characterization of recombinant human prolidase (PEPD) in Escherichia coli
**作者**：Yang Y, et al.
**摘要**：该研究成功在大肠杆菌中表达并纯化具有生物活性的人源PEPD重组蛋白，优化了表达条件，并通过酶活性实验证实其催化脯氨酸二肽水解的功能。
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2. **标题**：PEPD promotes EGFR-mediated signaling via modulating phosphorylation dynamics
**作者**：He X, et al.
**摘要**：研究发现重组PEPD蛋白通过调控EGFR的磷酸化状态激活下游MAPK信号通路，揭示PEPD在肿瘤微环境中潜在调控细胞增殖的作用机制。
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3. **标题**：Therapeutic potential of recombinant PEPD in collagen metabolism disorders
**作者**：Adhihetty PJ, et al.
**摘要**：通过动物模型验证重组PEPD蛋白可改善胶原代谢异常导致的肝纤维化表型，表明其作为代谢性疾病治疗药物的潜力。
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注：以上文献为示例性质，实际引用需根据具体研究内容核实原文。建议通过PubMed或Web of Science以关键词“recombinant PEPD/prolidase”检索最新研究。
**Background of Recombinant PEPD Protein**
PEPD (Prolidase), also known as peptidase D, is a manganese-dependent dipeptidase that specifically cleaves imidodipeptides containing C-terminal proline or hydroxyproline, playing a critical role in collagen metabolism and the recycling of proline for protein synthesis. This enzyme is essential for maintaining connective tissue integrity and cellular homeostasis. Mutations in the *PEPD* gene are linked to prolidase deficiency, a rare autosomal recessive disorder characterized by skin ulcers, recurrent infections, and developmental delays, highlighting its biological significance.
Recombinant PEPD protein is produced using biotechnological platforms, such as *E. coli* or mammalian expression systems, to ensure high purity and activity. Its production enables detailed studies of enzyme kinetics, substrate specificity, and structural biology, aiding in the development of therapeutic strategies for prolidase deficiency. Additionally, recombinant PEPD has garnered interest in industrial applications, including biocatalysis for peptide synthesis and degradation in biomanufacturing.
Recent research also explores PEPD's potential roles beyond collagen turnover. Studies suggest it may influence cellular processes like apoptosis, redox regulation, and even cancer progression, though these findings require further validation. The availability of recombinant PEPD facilitates drug discovery, biomarker research, and enzyme replacement therapy (ERT) development, offering hope for treating genetic and acquired disorders linked to prolidase dysfunction.
In summary, recombinant PEPD serves as a vital tool for both basic research and clinical applications, bridging gaps in understanding proline metabolism and advancing therapeutic innovations.
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