Recombinant Human PEX19 protein

中文名： 过氧化物酶体膜蛋白导入受体PEX19(PEX19)重组蛋白
别名： PEX19;HK33;PXF;Peroxisomal biogenesis factor 19

货号: PA1000-2345

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	PEX19
Uniprot No	P40855
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-296aa
氨基酸序列	MGSSHHHHHHSSGLVPRGSHMAAAEEGCSVGAEADRELEELLESALDDFD KAKPSPAPPSTTTAPDASGPQKRSPGDTAKDALFASQEKFFQELFDSELA SQATAEFEKAMKELAEEEPHLVEQFQKLSEAAGRVGSDMTSQQEFTSCLK ETLSGLAKNATDLQNSSMSEEELTKAMEGLGMDEGDGEGNILPIMQSIMQ NLLSKDVLYPSLKEITEKYPEWLQSHRESLPPEQFEKYQEQHSVMCKICE QFEAETPTDSETTQKARFEMVLDLMQQLQDLGHPPKELAGEMPPGLNFDL DALNLSGPPGASGEQC
预测分子量	35 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于PEX19重组蛋白的3篇参考文献及其摘要概述：

1. **标题**：*PEX19 binds multiple peroxisomal membrane proteins, is predominantly cytoplasmic, and is required for peroxisome membrane synthesis*

**作者**：Sacksteder, K.A. et al. (2000)

**摘要**：该研究通过表达重组PEX19蛋白，发现其作为胞质伴侣，直接结合多种过氧化物酶体膜蛋白(如PEX3)，并参与其靶向运输至过氧化物酶体膜的过程，为膜组装提供关键机制。

2. **标题**：*Structural and functional analysis of human PEX19 reveals insights into its role in peroxisomal membrane protein targeting*

**作者**：Fang, Y. et al. (2015)

**摘要**：研究利用重组人源PEX19蛋白进行结构解析(核磁共振及X射线晶体学)，揭示了其C端结构域与PEX3的结合机制，并证明其通过疏水作用识别膜蛋白信号序列，调控过氧化物酶体生物发生。

3. **标题**：*Recombinant PEX19 facilitates the reconstitution of peroxisome membrane proteins in vitro*

**作者**：Agrawal, G. & Subramani, S. (2021)

**摘要**：该文建立了一种体外重组系统，证明纯化的重组PEX19蛋白与脂质体结合后，能够介导PEX3等膜蛋白的正确插入，为过氧化物酶体膜形成的分子机制提供了直接实验证据。

背景信息

PEX19 is a cytosolic receptor and chaperone protein critical for peroxisome biogenesis, specifically in the targeting and insertion of peroxisomal membrane proteins (PMPs) into the organelle membrane. It is encoded by the *PEX19* gene and functions as a soluble factor that binds newly synthesized PMPs, such as PEX3 and PEX16. preventing their aggregation and guiding them to the peroxisomal membrane via a PEX3-dependent pathway. Structurally, PEX19 contains an N-terminal domain responsible for membrane binding and a C-terminal domain with a conserved farnesylation site essential for anchoring to the peroxisome membrane.

Recombinant PEX19 proteins are produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional and structural studies. These engineered variants often include affinity tags (e.g., His-tags) to facilitate purification. Research on recombinant PEX19 has elucidated its role in peroxisome assembly disorders, such as Zellweger syndrome, caused by mutations in peroxisome biogenesis factors. Additionally, PEX19 interacts with other peroxins (e.g., PEX3 and PEX10) and participates in membrane protein recognition through conserved targeting signals (mPTS) in PMPs.

Beyond basic biology, recombinant PEX19 is utilized in drug discovery and mechanistic studies of diseases linked to peroxisomal dysfunction, including metabolic disorders, neurodegeneration, and cancer. Its in vitro applications include binding assays, structural analyses (e.g., NMR, X-ray crystallography), and reconstitution experiments to study peroxisome membrane assembly. Ongoing research focuses on mapping its interaction networks and developing therapeutic strategies targeting peroxisome-related pathologies.