Rabbit Polyclonal GSTK1 Antibody

The glutathione S-transferase kappa 1 (GSTK1) antibody is a tool used to detect and study the GSTK1 protein, a member of the glutathione S-transferase (GST) family. GSTs are phase II detoxification enzymes that conjugate glutathione to xenobiotics, facilitating their elimination. Unlike other GST isoforms localized in the cytoplasm or microsomes, GSTK1 is uniquely targeted to mitochondria and peroxisomes due to its N-terminal organelle-targeting sequence. This mitochondrial/peroxisomal localization suggests specialized roles in oxidative stress regulation, lipid metabolism, and apoptosis. GSTK1 antibodies are typically raised against recombinant GSTK1 protein fragments or specific epitopes, often in hosts like rabbits or mice. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate GSTK1 expression patterns in tissues or cell lines. Research applications include studying mitochondrial dysfunction, metabolic disorders (e.g., diabetes, obesity), neurodegenerative diseases, and cancer, where GSTK1 dysregulation has been implicated. Commercial antibodies often undergo validation for specificity via knockout controls or siRNA knockdown. As GSTK1 may interact with redox-sensitive pathways, its antibody also serves as a marker for exploring cellular antioxidant mechanisms. Proper controls are essential due to potential cross-reactivity with other GST family members.