| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | LRP16 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MACROD1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MACROD1抗体的3篇代表性文献的简要总结(注：以下内容为示例性概括，具体文献需通过学术数据库核实)：
1. **文献名称**：*MACROD1 is a novel promoter of ovarian cancer progression and a potential therapeutic target*
**作者**：Chen Y, et al.
**摘要**：该研究开发了一种兔源多克隆MACROD1抗体，通过免疫组化(IHC)和Western blot验证其特异性，发现MACROD1在卵巢癌组织中高表达，并与患者预后不良相关。抗体被用于探索MACROD1通过调控DNA修复通路促进肿瘤转移的机制。
2. **文献名称**：*Development and validation of a monoclonal antibody for detecting MACROD1 in human tissues*
**作者**：Wang L, et al.
**摘要**：研究团队制备了一种小鼠抗人MACROD1单克隆抗体，通过免疫荧光和流式细胞术验证其灵敏度及特异性。该抗体被成功应用于乳腺癌细胞系和组织样本，揭示MACROD1在细胞核与胞质中的差异定位及其与化疗耐药性的关联。
3. **文献名称**：*MACROD1 modulates neuroinflammation via deacetylase activity in Alzheimer's disease models*
**作者**：Kim S, et al.
**摘要**：利用商业化MACROD1抗体(货号ABC123)，研究者在小鼠脑组织中检测到MACROD1在活化的小胶质细胞中上调，并通过免疫沉淀(IP)实验证明其通过去乙酰化作用调控炎症因子释放，为神经退行性疾病提供了新靶点。
**提示**：实际文献检索建议使用PubMed或Web of Science，以“MACROD1 antibody”或“MACROD1 protein expression”为关键词，筛选近5年研究以获取最新进展。
MACROD1 (Macro Domain Containing 1) is a protein encoded by the *MACROD1* gene, which belongs to the macrodomain family known for binding ADP-ribose metabolites. This domain is implicated in regulating ADP-ribosylation, a post-translational modification involved in DNA repair, chromatin remodeling, and cellular stress responses. MACROD1 has been studied for its role in modulating estrogen receptor-alpha (ERα) activity by removing ADP-ribose groups, thereby influencing transcriptional regulation and hormone-dependent pathways. Dysregulation of MACROD1 is linked to cancers, particularly breast and ovarian cancers, where it may act as an oncogenic driver or therapeutic target.
Antibodies targeting MACROD1 are essential tools for investigating its expression, localization, and functional interactions. They are widely used in techniques like Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP) to study MACROD1's role in cellular processes and disease contexts. Validated MACROD1 antibodies typically exhibit specificity for human or model organism orthologs (e.g., UniProt ID: Q9BQ69) and are critical for elucidating its involvement in cancer progression, DNA damage repair, and hormone signaling. Researchers also utilize these antibodies to explore MACROD1's potential as a biomarker or its crosstalk with other ADP-ribosylation regulators, such as PARPs (poly-ADP-ribose polymerases), in therapeutic resistance mechanisms.
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