| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | AAKG; CMH6; WPWS; AAKG2; H91620p |
| WB Predicted band size | 63 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human PRKAG2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与PRKAG2抗体相关的文献示例(注：文献为虚拟示例，实际引用需核实真实来源)：
1. **"PRKAG2 Cardiac Syndrome: Characterization of a Transgenic Mouse Model"**
- **作者**: Smith A, et al.
- **摘要**: 利用PRKAG2特异性抗体，通过Western blot和免疫组化分析转基因小鼠心脏组织中PRKAG2蛋白的表达及定位，揭示其突变导致糖原累积和心肌肥厚的分子机制。
2. **"AMPK γ2 Subunit Mutations and Hypertrophic Cardiomyopathy"**
- **作者**: Johnson R, et al.
- **摘要**: 研究PRKAG2基因突变与家族性肥厚型心肌病的关系，采用抗PRKAG2抗体检测患者心肌活检样本中的蛋白异常表达，发现突变体导致AMPK活性改变及能量代谢紊乱。
3. **"Role of PRKAG2 in Cellular Energy Sensing: Insights from Knockout Models"**
- **作者**: Lee C, et al.
- **摘要**: 通过构建PRKAG2敲除小鼠，结合抗体介导的蛋白表达分析，证实PRKAG2在调控AMPK复合体活性及糖原代谢中的关键作用，并探索其与代谢综合征的关联。
4. **"Immunohistochemical Analysis of PRKAG2 in Human Skeletal Muscle"**
- **作者**: Garcia M, et al.
- **摘要**: 使用特异性PRKAG2抗体研究人类骨骼肌组织中该蛋白的分布，发现其在运动后能量代谢适应中的动态变化，提示其与运动诱导的AMPK信号通路激活相关。
(注：以上文献信息为示例，实际研究中需引用真实文献。)
The PRKAG2 antibody is a research tool designed to detect and study the PRKAG2 protein, a regulatory subunit of the adenosine monophosphate-activated protein kinase (AMPK) complex. AMPK is a critical energy sensor in eukaryotic cells, regulating metabolic homeostasis by responding to changes in ATP levels. The PRKAG2 subunit (γ2 isoform) plays a key role in modulating AMPK activity by binding adenosine nucleotides (AMP, ADP, ATP), which influences the enzyme’s ability to phosphorylate downstream targets involved in glucose uptake, lipid metabolism, and mitochondrial biogenesis.
Mutations in the PRKAG2 gene are linked to cardiac syndromes such as hypertrophic cardiomyopathy, Wolff-Parkinson-White (WPW) syndrome, and glycogen storage disease, making the PRKAG2 protein a focus in cardiovascular and metabolic research. Antibodies targeting PRKAG2 enable the detection of its expression, localization, and post-translational modifications in tissues and cell lines. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate its role in disease models, AMPK signaling pathways, and therapeutic interventions.
Commercial PRKAG2 antibodies are typically raised against specific epitopes, often validated for cross-reactivity in human, mouse, or rat samples. Researchers rely on these antibodies to dissect PRKAG2’s regulatory mechanisms and its contribution to pathologies, aiding in the development of targeted therapies for AMPK-related disorders.
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