首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠过氧化氢酶(CAT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CAT含量。靶点研究是医药领域的关键环节，通过深入了解疾病分子机制和生物学特性，寻找新的药物靶点，设计精确高效的药物治疗方案。研究机制包括基因组学、蛋白质组学和生物信息学，以及高通量筛选技术。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外培养细胞模型中的抗氧化能力评估、动物实验中组织氧化应激水平分析，以及疾病模型（如代谢综合征、神经退行性疾病）中氧化损伤机制的探索性研究，为生命科学基础研究提供可靠的检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cat ELISA kit; Cas-1 ELISA kit; Cas1Catalase ELISA kit; EC 1.11.1.6 ELISA kit
缩写：CAT
Uniprot No.：P24270
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:2000 | Average % | 93 | ||||
| Range % | 84-99 | |||||
| 1:4000 | Average % | 98 | ||||
| Range % | 94-106 | |||||
| 1:8000 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of mouse CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4032.4532.4282.2915002.1782.1962.1872.0502501.7451.7871.7661.6291251.2171.3091.2631.12662.50.7610.7780.7700.63331.20.4820.4990.4910.35415.60.3120.3330.3230.18600.1380.1360.137 | ||||||
货期：3-5 working days
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