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产品描述：猪胆囊收缩素/肠促胰酶肽(CCK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCK含量。CCK即缩胆囊素，是一种胃肠道激素，在神经系统也作为神经递质存在。其广泛参与消化调节、摄食调控等生理过程。研究机制多聚焦于与CCK受体结合，激活下游信号通路，在消化、神经等领域开展研究，助力相关疾病治疗策略探索。试剂盒检测范围为1.56 pg/mL-100 pg/mL，该产品适用于科研领域中对猪源性样本CCK表达水平的定量研究，例如动物消化系统功能评估、神经肽作用机制探索或代谢调控相关实验。通过兼容血清、血浆及多种组织样本的检测特性，本试剂盒为研究者提供灵活的实验方案选择，助力于猪模型在胃肠生理、神经生物学及代谢疾病机制等方向的科研工作。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CCK ELISA Kit; Cholecystokinin ELISA Kit; CCK) [Cleaved into: Cholecystokinin-58 ELISA Kit; CCK58); Cholecystokinin-58 desnonopeptide ELISA Kit; (1-49)-CCK58); Cholecystokinin-39 ELISA Kit; CCK39); Cholecystokinin-33 ELISA Kit; CCK33); Cholecystokinin-25 ELISA Kit; CCK25); Cholecystokinin-18 ELISA Kit; CCK18); Cholecystokinin-12 ELISA Kit; CCK12); Cholecystokinin-8 ELISA Kit; CCK8); Cholecystokinin-7 ELISA Kit; CCK7); Cholecystokinin-5 ELISA Kit; CCK5)] ELISA Kit
缩写：CCK
Uniprot No.：P01356
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 93-100 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 101-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 87-93 | |||||
回收率：
| The recovery of pig CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-89 | ||||
| EDTA plasma (n=4) | 92 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.1792.1982.1892.031501.6981.6771.6881.530251.1361.1661.1510.99312.50.6970.6580.6780.5206.250.4860.4970.4920.3343.120.3830.3980.3910.2331.560.3010.3210.3110.15300.1570.1590.158 | ||||||
货期：3-5 working days
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