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Rabbit Polyclonal POLD2 Antibody

  • 中文名: POLD2抗体
  • 别    名: nan
货号: IPDX10178
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/300 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

WB Predicted band size51 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenFusion protein of human POLD2
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是3篇关于POLD2抗体的模拟参考文献示例(注:文献为假设性示例,实际引用请核实真实来源):

1. **文献名称**: "POLD2 as a Prognostic Biomarker in Colorectal Cancer: Immunohistochemical Analysis"

**作者**: Zhang Y. et al.

**摘要**: 本研究通过免疫组化(IHC)技术,使用POLD2特异性抗体检测结直肠癌组织中POLD2蛋白的表达水平,发现其高表达与患者预后不良显著相关,提示POLD2可能作为癌症进展的潜在标志物。

2. **文献名称**: "Role of POLD2 in DNA Damage Repair: Insights from siRNA and Antibody-Based Knockdown"

**作者**: Lee S. et al.

**摘要**: 通过Western blot和免疫荧光技术结合POLD2抗体,研究证实POLD2在DNA损伤修复中的关键作用。实验显示,抑制POLD2表达导致细胞对电离辐射敏感性增加,揭示其作为治疗靶点的潜力。

3. **文献名称**: "POLD2 Antibody Validation for Flow Cytometry in Cell Cycle Studies"

**作者**: Smith J. et al.

**摘要**: 本文系统验证了POLD2抗体在流式细胞术中的应用,证明其特异性识别细胞周期S期中的POLD2蛋白,为研究DNA复制调控提供了可靠工具。

4. **文献名称**: "POLD2 Interaction Network Revealed by Co-Immunoprecipitation Assays"

**作者**: Gupta R. et al.

**摘要**: 利用POLD2抗体进行免疫共沉淀(Co-IP)实验,鉴定出POLD2与多个DNA修复复合体的相互作用蛋白,阐明其在维持基因组稳定性中的分子机制。

(注:以上为模拟文献,实际研究中请通过PubMed或Google Scholar以关键词“POLD2 antibody”、“DNA polymerase delta subunit 2”检索真实文献。)

背景信息

The POLD2 antibody is a crucial tool for studying the DNA polymerase delta subunit 2 (POLD2), a vital component of the DNA polymerase delta complex. This enzyme plays a central role in eukaryotic DNA replication and repair, particularly in synthesizing and proofreading lagging-strand DNA during replication. POLD2. as the second regulatory subunit, stabilizes the complex and modulates its enzymatic activity, ensuring genomic integrity. Researchers use POLD2 antibodies to investigate its expression, localization, and interaction with other replication/repair proteins in cellular processes like cell cycle progression, DNA damage response, and telomere maintenance. These antibodies are widely applied in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to assess POLD2 levels in cancer studies, as dysregulation of DNA replication machinery is linked to tumorigenesis and genomic instability. Commercially available POLD2 antibodies are typically validated for specificity in human, mouse, or rat samples, aiding mechanistic studies of replication errors, aging-related disorders, and targeted therapies. Understanding POLD2's role through antibody-based assays contributes to insights into diseases like cancer, developmental syndromes, and chemotherapy resistance mechanisms.

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