首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠CD63抗原(CD63)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD63含量。CD63 是一种广泛存在于细胞膜表面的糖蛋白，属于四次跨膜蛋白超家族成员。它参与细胞黏附、迁移等多种生理过程。在研究机制方面，它可作为细胞外泌体标志物，还与肿瘤转移、免疫调节等密切相关，是当前生物医学领域的研究热点之一。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于体外科研实验中检测CD63的表达水平变化，例如探究神经退行性疾病中的外泌体释放、肿瘤微环境中细胞互作机制或免疫细胞活化相关研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cd63CD63 antigen ELISA kit; CD antigen CD63 ELISA kit
缩写：CD63
Uniprot No.：P41731
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD63 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-104 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 95-105 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of mouse CD63 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 98-107 | ||||
| EDTA plasma (n=4) | 94 | 90-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.1042.2032.1542.01320001.5541.4551.5051.36410000.8530.8820.8680.7275000.4960.5050.5010.3602500.3260.3170.3220.1811250.2350.2440.2400.09962.50.1780.1750.1770.03600.1400.1410.141 | ||||||
货期：3-5 working days
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