| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | LCA14 |
| WB Predicted band size | 26 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human LRAT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与LRAT(卵磷脂视黄醇酰基转移酶)抗体相关的研究文献，简要总结其核心内容：
1. **"Lecithin retinol acyltransferase is essential for accumulation of all-trans-retinyl esters in the eye and in the liver"**
*作者：Batten ML 等*
摘要：通过生成LRAT基因敲除小鼠模型，利用特异性抗体检测发现LRAT缺失导致视网膜和肝脏中全反式视黄酯完全缺失，证实LRAT是维生素A储存的关键酶。
2. **"Immunohistochemical localization of lecithin retinol acyltransferase in the retina and retinal pigment epithelium"**
*作者：Ruiz A 等*
摘要：使用LRAT特异性抗体进行免疫组化分析，揭示该酶在视网膜色素上皮细胞中的极性分布，提示其参与视觉周期中视黄醇的定向转运。
3. **"LRAT coordinates the negative-feedback regulation of hepatic retinoid metabolism"**
*作者：Kawaguchi R 等*
摘要：通过Western blot和免疫荧光技术，发现肝脏星状细胞中LRAT蛋白水平受维生素A状态动态调节，抗体检测证实其在维持视黄酸稳态中的负反馈作用。
4. **"Retinol esterification by LRAT is required for dietary retinoid absorption"**
*作者：Ong DE 等*
摘要：利用抗体验证小肠上皮细胞LRAT表达，发现其通过酯化膳食视黄醇促进乳糜微粒组装，抗体阻断实验进一步证明该酶在肠道维生素A吸收中的必要性。
**Background of LRAT Antibodies**
LRAT (lecithin retinol acyltransferase) is a key enzyme in the visual cycle and vitamin A metabolism, catalyzing the esterification of all-*trans*-retinol into all-*trans*-retinyl esters within retinal pigment epithelial (RPE) cells. This reaction is critical for storing retinoids and maintaining retinal homeostasis. Dysregulation of LRAT activity is linked to retinal disorders like Stargardt disease, Leber congenital amaurosis, and age-related macular degeneration (AMD).
LRAT antibodies are immunological tools designed to detect and quantify LRAT protein expression in research and diagnostic settings. These antibodies are typically generated using peptide antigens derived from conserved regions of the human or murine LRAT protein. They enable the study of LRAT's role in retinoid metabolism, cellular localization in RPE cells, and its interaction with other visual cycle components.
In research, LRAT antibodies are utilized in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess LRAT expression levels in disease models or genetic studies. For instance, they help evaluate LRAT deficiency in knockout mice or analyze LRAT upregulation in therapeutic interventions targeting retinal degeneration. Commercially available LRAT antibodies are often validated for specificity and sensitivity, though variability in performance across species or tissue types requires careful optimization.
Beyond basic research, LRAT antibodies hold potential in developing biomarker assays for retinal diseases and monitoring gene therapy outcomes aimed at restoring LRAT function in inherited blindness. Their application bridges mechanistic insights into vitamin A metabolism and translational efforts to treat LRAT-associated pathologies.
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