首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠胆碱乙酰转移酶（ChAT）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的CHAT含量。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，在神经退行性疾病模型研究、神经发育机制探索及药物干预效果评估中具有重要科研价值。为神经科学、药理学及基础医学研究提供稳定高效的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ChatCholine O-acetyltransferase ELISA kit; CHOACTase ELISA kit; ChAT ELISA kit; Choline acetylase ELISA kit; EC 2.3.1.6 ELISA kit
缩写：CHAT
Uniprot No.：Q03059
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ChAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:200 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:400 | Average % | 103 | |||||
| Range % | 99-108 | ||||||
| 1:800 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
回收率：
| The recovery of mouse ChAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 91-100 | |||||
| EDTA plasma (n=4) | 92 | 88-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.0422.1362.0891.9335001.4071.4811.4441.2882500.8930.9470.9200.7641250.6100.6580.6340.47862.50.4750.4630.4690.31331.20.3210.3050.3130.15715.60.2540.2490.2520.09600.1590.1520.156 | |||||||
货期：3-5 working days
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