| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Cd36 |
| Uniprot No | P16671 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-439aa |
| 氨基酸序列 | DLLIQKTIKKQVVLEEGTIAFKNWVKTGTEVYRQFWIFDVQNPQEVMMNS SNIQVKQRGPYTYRVRFLAKENVTQDAEDNTVSFLQPNGAIFEPSLSVGT EADNFTVLNLAVAAASHIYQNQFVQMILNSLINKSKSSMFQVRTLRELLW GYRDPFLSLVPYPVTTTVGLFYPYNNTADGVYKVFNGKDNISKVAIIDTY KGKRNLSYWESHCDMINGTDAASFPPFVEKSQVLQFFSSDICRSIYAVFE SDVNLKGIPVYRFVLPSKAFASPVENPDNYCFCTEKIISKNCTSYGVLDI SKCKEGRPVYISLPHFLYASPDVSEPIDGLNPNEEEHRTYLDIEPITGFT LQFAKRLQVNLLVKPSEKIQVLKNLKRNYIVPILWLNETGTIGDEKANMF RSQVTGKIN |
| 预测分子量 | 53 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Purification and functional characterization of recombinant CD36"**
- Authors: Smith A, et al.
- 摘要：通过哺乳动物表达系统成功表达并纯化CD36重组蛋白，验证其与氧化低密度脂蛋白(oxLDL)的结合活性，揭示其在泡沫细胞形成中的潜在作用机制。
2. **"Structural insights into CD36-mediated lipid recognition"**
- Authors: Chen L, et al.
- 摘要：利用重组CD36蛋白进行晶体结构解析，阐明其脂质结合域的三维构象，发现特定氨基酸残基对脂肪酸和磷脂的识别至关重要。
3. **"CD36 recombinant protein attenuates inflammation in sepsis models"**
- Authors: Wang Y, et al.
- 摘要：研究重组CD36蛋白在小鼠脓毒症模型中的抗炎作用，证明其通过抑制TLR4/NF-κB信号通路减少促炎因子释放。
4. **"Recombinant CD36 enhances β-cell function via fatty acid uptake regulation"**
- Authors: Gupta R, et al.
- 摘要：体外实验表明，添加CD36重组蛋白可调节胰岛β细胞的脂肪酸摄取，改善脂毒性诱导的胰岛素分泌功能障碍，为糖尿病治疗提供新思路。
(注：上述文献为模拟内容，实际引用需根据具体论文调整。)
CD36. also known as cluster of differentiation 36 or fatty acid translocase (FAT), is a multifunctional transmembrane glycoprotein belonging to the class B scavenger receptor (SR-B) family. It plays critical roles in lipid metabolism, angiogenesis, immune response, and cellular signaling by interacting with diverse ligands, including oxidized low-density lipoprotein (oxLDL), long-chain fatty acids, thrombospondin-1. and amyloid-β. CD36 is expressed in various cell types, such as macrophages, adipocytes, endothelial cells, and platelets, and its dysregulation is implicated in metabolic disorders, atherosclerosis, diabetes, and cancer.
Recombinant CD36 protein is engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional CD36 for research and therapeutic applications. The recombinant form typically retains key structural features, such as the extracellular domain with ligand-binding sites, transmembrane regions, and post-translational modifications (e.g., glycosylation) when expressed in eukaryotic systems. Its production enables mechanistic studies of CD36-mediated processes—lipid uptake, apoptosis, phagocytosis, and inflammatory signaling—in controlled experimental settings.
Researchers utilize recombinant CD36 to investigate its role in disease pathogenesis, screen potential inhibitors or agonists, and develop diagnostic tools. For example, it aids in studying CD36's contribution to foam cell formation in atherosclerosis or its interaction with pathogens like *Plasmodium falciparum* in malaria. Challenges in production include maintaining protein stability and native conformation, particularly for membrane-associated domains. Despite this, recombinant CD36 remains a vital tool for unraveling its complex biology and exploring therapeutic strategies targeting metabolic and inflammatory diseases.
×
