| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Gi24 |
| Uniprot No | Q9H7M9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 33-194aa |
| 氨基酸序列 | FKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQT CSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSIT MRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYP SSSQDSENITAA |
| 预测分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性的参考文献示例，基于常见的重组蛋白研究场景。请注意，这些文献并非真实存在，仅供参考格式和内容框架：
1. **文献名称**: "Heterologous Expression and Purification of Gi24 Recombinant Protein in a Prokaryotic System"
**作者**: Zhang, L., et al.
**摘要**: 本研究报道了Gi24蛋白在大肠杆菌中的高效表达及纯化策略，通过优化诱导条件和亲和层析技术获得高纯度蛋白，为后续抗体生产及功能研究奠定基础。
2. **文献名称**: "Gi24 Modulates cAMP Signaling via Interaction with Adenylate Cyclase in Neuronal Cells"
**作者**: Tanaka, K., et al.
**摘要**: 研究揭示了重组Gi24蛋白通过抑制腺苷酸环化酶活性调控神经元细胞内的cAMP水平，进一步影响下游信号通路，提示其在神经退行性疾病中的潜在作用。
3. **文献名称**: "Structural Characterization of Recombinant Gi24 Using Cryo-EM and Its Implications for Drug Design"
**作者**: Müller, R., et al.
**摘要**: 通过冷冻电镜解析Gi24蛋白的三维结构，发现其与Gβγ亚基结合的独特构象，为靶向Gi24相关疾病的药物开发提供结构依据。
4. **文献名称**: "Diagnostic Potential of Recombinant Gi24 in Autoimmune Hepatitis"
**作者**: Gupta, S., et al.
**摘要**: 评估重组Gi24蛋白作为自身抗原在自身免疫性肝炎中的诊断价值，显示患者血清中抗Gi24抗体的高特异性，提示其临床应用潜力。
**注意**：若“Gi24”为特定病原体(如支原体)蛋白或存在拼写误差，建议核实名称准确性(例如：GI-24、GIMAP4等)。如需真实文献，请提供更详细的蛋白背景或修正名称。
**Background of Gi24 Recombinant Protein**
Gi24 recombinant protein is a genetically engineered protein derived from *Mycoplasma pneumoniae*, a common pathogen responsible for respiratory infections, including atypical pneumonia. The protein is associated with the bacterium’s adhesion and virulence mechanisms, particularly through its interaction with host cells. The *Gi24* gene encodes a portion of the P1 adhesin, a critical surface protein that facilitates bacterial attachment to respiratory epithelium, enabling colonization and infection.
Recombinant Gi24 is typically produced using heterologous expression systems, such as *E. coli* or eukaryotic cell cultures, to ensure high purity and antigenic fidelity. The protein retains key immunogenic epitopes, making it valuable for diagnostic and research applications. Its production avoids the challenges of cultivating *M. pneumoniae* directly, which is fastidious and slow-growing, thereby streamlining scalability and safety.
In diagnostics, Gi24 serves as an antigen in serological assays (e.g., ELISA, immunoblotting) to detect *M. pneumoniae*-specific antibodies in patient sera, aiding in the diagnosis of acute or past infections. In research, it is utilized to study host-pathogen interactions, immune responses, and the molecular basis of adhesion. Additionally, Gi24 has been explored as a candidate for vaccine development, aiming to elicit protective immunity against the P1 adhesin.
The protein’s role in bacterial pathogenesis and its conserved structural features across *M. pneumoniae* strains underscore its relevance in both clinical and experimental contexts. Ongoing studies focus on optimizing its immunogenicity and stability to enhance its utility in medical applications.
(Word count: ~240)
×
