| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLE3 |
| Uniprot No | Q9NRF9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-147aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAERPED LNLPNAVITR IIKEALPDGV NISKEARSAI SRAASVFVLY ATSCANNFAM KGKRKTLNAS DVLSAMEEME FQRFVTPLKE ALEAYRREQK GKKEASEQKK KDKDKKTDSE EQDKSRDEDN DEDEERLEEE EQNEEEEVDN |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与POLE3重组蛋白相关的参考文献及摘要概括：
1. **"Structure and function of the histone chaperone CIA/ASF1 complexed with histones H3 and H4"**
*Authors: Antczak et al.*
摘要：研究解析了POLE3(CIA)与组蛋白H3-H4复合物的晶体结构，阐明其通过重组蛋白技术揭示POLE3在染色质组装中的分子机制，证明其作为组蛋白伴侣的功能。
2. **"DNA polymerase ε relies on a unique domain for efficient replisome assembly and DNA synthesis"**
*Authors: Hogg et al.*
摘要：通过重组表达POLE3蛋白，证明其与POLE4形成稳定复合物，对DNA聚合酶ε的复制叉定位和复制起始至关重要，并揭示其缺失导致基因组不稳定性。
3. **"The human CTF4-orthologue AND-1 interacts with DNA polymerase α/primase via its conserved C-terminal domain"**
*Authors: Im et al.*
摘要：利用重组POLE3蛋白进行体外互作实验，发现其通过C端结构域与复制解旋酶复合物结合，协调复制过程中前导链与滞后链的协同合成。
注：POLE3相关研究多集中于其作为DNA复制复合物亚基的功能及结构解析，上述文献涵盖结构生物学、复制机制及分子互作方向。若需具体文献DOI或补充研究，可进一步提供方向细化。
POLE3. also known as C17orf37 or CHRAC17. is a subunit of the DNA polymerase epsilon (Pol ε) complex, a key multi-protein assembly involved in eukaryotic DNA replication and repair. As part of the B-family DNA polymerases, Pol ε primarily contributes to leading-strand synthesis during replication. POLE3. along with its binding partner POLE4. forms a stable heterodimer that associates with the catalytic subunit POL2 (POLE1) and the accessory subunit POLE2. This structural arrangement enables Pol ε to coordinate polymerase activity with chromatin remodeling and replication fork progression.
Functionally, POLE3 plays a regulatory role in stabilizing the Pol ε complex and modulating its enzymatic activity. It has been implicated in facilitating replication origin firing by interacting with chromatin remodelers like the histone chaperone complex FACT (Facilitates Chromatin Transcription). Additionally, POLE3 contributes to epigenetic regulation through its association with histone H3-H4 tetramers, influencing nucleosome assembly during replication. Emerging studies suggest its involvement in replication stress responses and genome stability maintenance, linking dysregulation of POLE3 to oncogenesis and developmental disorders.
Recombinant POLE3 protein is engineered for in vitro studies to dissect its molecular interactions, enzymatic roles, and structural contributions to the Pol ε complex. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), it often includes affinity tags (e.g., His-tag) for purification. Research utilizing recombinant POLE3 has advanced understanding of replication mechanisms, chromatin dynamics, and potential therapeutic targets in cancers with replication-related mutations. Its study remains critical for elucidating fundamental processes in DNA metabolism and disease pathology.
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