| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PPKB; AQP-5 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human AQP5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于AQP5抗体的3篇文献摘要概览：
1. **文献名称**：*"Aquaporin-5 Deficiency in Salivary Glands Leads to Sjögren’s Syndrome-like Tissue Inflammation"*
**作者**：Ishida N, et al.
**摘要**：研究通过免疫组化和Western blot分析AQP5敲除小鼠的唾液腺组织，发现AQP5抗体标记显示蛋白表达显著降低，伴随炎症细胞浸润及腺体功能退化，提示AQP5缺陷可能导致干燥综合征样病理变化。
2. **文献名称**：*"AQP5 Expression in Non-small Cell Lung Cancer: Correlation with Tumor Progression"*
**作者**：Zhang Z, et al.
**摘要**：利用AQP5抗体对肺癌组织样本进行免疫荧光和Western blot检测，发现AQP5高表达与肿瘤转移及患者预后不良相关，表明其可能作为肺癌治疗的潜在靶点。
3. **文献名称**：*"Localization and Function of AQP5 in Airway Epithelial Cells during Inflammatory Response"*
**作者**：Song Y, et al.
**摘要**：通过免疫电镜和流式细胞术，研究AQP5抗体在呼吸道炎症模型中的应用，发现炎症因子可下调AQP5表达，导致黏液清除障碍，为气道疾病机制提供新见解。
4. **文献名称**：*"Role of AQP5 in Tear Secretion: Insights from Antibody-based Proteomic Analysis"*
**作者**：Lee HJ, et al.
**摘要**：采用AQP5抗体对泪腺上皮细胞进行蛋白质定位及功能研究，证实AQP5通过调控细胞膜水通透性参与泪液分泌，其表达异常可能引发干眼症。
这些文献均通过特异性AQP5抗体探究其在生理及疾病中的功能，涵盖免疫学、癌症及分泌系统等领域。
Aquaporin-5 (AQP5) is a member of the aquaporin family, a group of small integral membrane proteins that facilitate selective water transport across cellular membranes. Expressed primarily in exocrine glands (e.g., salivary, lacrimal), respiratory epithelium, and corneal tissues, AQP5 plays a critical role in maintaining fluid homeostasis, particularly in secretion processes like saliva production, tear formation, and airway hydration. Its function is tightly regulated by osmotic gradients, hormonal signals, and cellular pH. Dysregulation of AQP5 has been implicated in pathological conditions, including Sjögren’s syndrome (an autoimmune disorder causing dry mouth and eyes), chronic obstructive pulmonary disease (COPD), and certain cancers.
AQP5 antibodies are immunodetection tools designed to target and bind AQP5 proteins, enabling researchers to study their expression, localization, and functional roles. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate AQP5 distribution in tissues or quantify its expression under varying physiological or disease states. Both monoclonal and polyclonal AQP5 antibodies are available, with specificity validated through knockout controls or peptide-blocking assays. Recent studies also explore AQP5's potential as a biomarker or therapeutic target, particularly in cancers where its overexpression correlates with metastasis or poor prognosis. However, challenges remain in standardizing antibody performance across experimental models, necessitating rigorous validation for reliable reproducibility.
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