| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLR2C |
| Uniprot No | P19387 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-275aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMPYANQPTVRITELTDENVKFIIENTD LAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGLIPLISDD IVDKLQYSRDCTCEEFCPECSVEFTLDVRCEDQTRHVTSRDLISNSRVIP VTSRNRDNDPNDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPT AGVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQAPYDPNGKPERF YYNVESCGSLRPETIVLSALSGLKKKLSDLQTQLSHEIQSDVLTIN |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于POLR2C重组蛋白的3篇代表性文献示例(注：文献为假设性概括，实际研究需根据具体论文调整)：
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1. **文献名称**：*Structural Insights into the Human RNA Polymerase II Subunit POLR2C*
**作者**：Smith A et al.
**摘要**：本研究通过重组表达人源POLR2C蛋白，解析其晶体结构，揭示了其与RNA聚合酶II核心复合体的相互作用界面，为理解真核生物转录机制提供结构基础。
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2. **文献名称**：*Functional Characterization of Recombinant POLR2C in Transcription Regulation*
**作者**：Zhang L et al.
**摘要**：利用重组POLR2C蛋白进行体外转录实验，证明该亚基对RNA聚合酶II的酶活性和启动子识别具有调控作用，并发现其磷酸化修饰影响转录延伸效率。
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3. **文献名称**：*POLR2C Recombinant Protein Interaction Network in Cancer Cells*
**作者**：Chen H et al.
**摘要**：通过重组POLR2C蛋白的亲和纯化与质谱分析，鉴定了其在癌细胞中与转录因子(如MYC)的相互作用网络，提示POLR2C可能参与肿瘤相关基因的异常表达。
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如需具体文献，建议在PubMed或Web of Science中检索关键词："POLR2C recombinant protein"、"RNA polymerase II subunit RPB3"(POLR2C别称)或结合研究领域(如结构、癌症、转录调控)。
**Background of POLR2C Recombinant Protein**
POLR2C (RNA Polymerase II Subunit C), also known as RPB3. is a critical component of the RNA polymerase II (RNAP II) complex, which is responsible for synthesizing messenger RNA (mRNA) in eukaryotic cells. This 35 kDa protein is encoded by the *POLR2C* gene and forms part of the core RNAP II structure, playing a central role in transcription initiation and elongation. POLR2C interacts with other subunits, such as RPB1 and RPB2. to stabilize the polymerase complex and ensure its proper assembly.
Recombinant POLR2C protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. Its recombinant form enables studies on RNAP II assembly, transcriptional regulation, and molecular mechanisms underlying diseases linked to dysregulated transcription, such as cancer or neurodegenerative disorders.
POLR2C’s structural and functional significance has made it a focus in drug discovery, particularly in targeting transcription-related pathologies. Recombinant POLR2C is also used in *in vitro* assays to investigate interactions with transcription factors, regulatory proteins, or potential inhibitors. Additionally, it aids in structural biology studies (e.g., X-ray crystallography or cryo-EM) to resolve RNAP II architecture and dynamics.
Mutations or altered expression of POLR2C have been associated with developmental defects and oncogenesis, highlighting its biological relevance. By providing a controllable and scalable source of the protein, recombinant POLR2C accelerates mechanistic insights into transcription while supporting therapeutic development.
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