| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLR2D |
| Uniprot No | O15514 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-142aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAAGGSD PRAGDVEEDA SQLIFPKEFE TAETLLNSEV HMLLEHRKQQ NESAEDEQEL SEVFMKTLNY TARFSRFKNR ETIASVRSLL LQKKLHKFEL ACLANLCPET AEESKALIPS LEGRFEDEEL QQILDDIQTK RSFQY |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于POLR2D重组蛋白的示例参考文献(内容为模拟生成，仅供参考)：
1. **文献名称**：Structural insights into the human RNA polymerase II subunit POLR2D
**作者**：Martinez-Rucobo, F.W., et al.
**摘要**：该研究通过冷冻电镜解析了POLR2D在RNA聚合酶II全酶复合体中的三维结构，揭示了其与亚基POLR2A的相互作用界面，并探讨了其在转录起始和延伸中的潜在调控作用。
2. **文献名称**：POLR2D overexpression promotes tumorigenesis by dysregulating transcription in breast cancer
**作者**：Li, X., et al.
**摘要**：研究发现POLR2D在乳腺癌细胞中异常高表达，通过干扰RNA聚合酶II的转录保真性导致促癌基因异常激活，提示POLR2D可能作为癌症治疗的潜在靶点。
3. **文献名称**：Recombinant expression and functional characterization of human POLR2D in E. coli
**作者**：Zhang, Y., et al.
**摘要**：本文成功在大肠杆菌中表达并纯化重组POLR2D蛋白，通过体外结合实验验证其与POLR2A的相互作用，为后续结构功能研究提供高效制备方案。
4. **文献名称**：POLR2D interacts with the histone acetyltransferase p300 to regulate gene-specific transcription
**作者**：Gupta, S., et al.
**摘要**：该研究利用免疫共沉淀技术发现POLR2D与表观修饰因子p300存在直接相互作用，并证明此互作在炎症相关基因的转录激活中起关键作用。
**注意**：以上文献为示例，实际文献请通过PubMed、Web of Science或Google Scholar等平台检索关键词“POLR2D”、“RNA polymerase II subunit 4”或“recombinant POLR2D protein”获取。
**Background of POLR2D Recombinant Protein**
POLR2D (RNA Polymerase II Subunit D) is a critical component of the RNA polymerase II (Pol II) complex, responsible for synthesizing messenger RNA (mRNA) in eukaryotic cells. As part of the core 12-subunit Pol II enzyme, POLR2D contributes to the structural and functional integrity of the catalytic center. It interacts with other core subunits (e.g., POLR2A, POLR2B) to stabilize the transcription machinery, facilitate DNA unwinding, and ensure accurate transcription initiation and elongation. POLR2D is evolutionarily conserved and plays a role in regulating transcription fidelity, stress responses, and cell cycle progression.
Recombinant POLR2D protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells*) to produce a purified, functional form for *in vitro* studies. Its recombinant version often includes affinity tags (e.g., His, GST) for simplified purification and detection. Researchers use this protein to investigate Pol II assembly, transcription mechanisms, and interactions with transcription factors or regulatory proteins. Dysregulation of POLR2D has been linked to diseases such as cancers and neurodegenerative disorders, making it a target for therapeutic exploration.
The availability of recombinant POLR2D enables structural studies (e.g., X-ray crystallography, cryo-EM), biochemical assays, and drug screening. Its role in transcription-coupled DNA repair and gene expression regulation underscores its importance in basic and applied biomedical research.
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