| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | BNP; Iso-ANP |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human NPPB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NPPB(B型利钠肽)抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*"B-type natriuretic peptide in cardiovascular disease"*
**作者**：Yasue H, Yoshimura M
**摘要**：该综述探讨了B型利钠肽(BNP/NPPB)在心血管疾病(如心力衰竭和心肌梗死)中的病理生理作用，并重点讨论了基于NPPB抗体的检测方法在临床诊断和预后评估中的应用价值。
2. **文献名称**：*"Development of a sensitive ELISA for human BNP using monoclonal antibodies"*
**作者**：Smith A, Jones B, et al.
**摘要**：研究团队开发了一种高灵敏度的ELISA检测方法，使用特异性NPPB单克隆抗体定量人血浆中的BNP水平，验证了其在心力衰竭患者中的诊断准确性，并与其他检测技术进行了对比分析。
3. **文献名称**：*"Targeted disruption of the BNP gene in mice reveals its physiological role in cardiac remodeling"*
**作者**：Tamura N, Ogawa Y, et al.
**摘要**：通过基因敲除小鼠模型，研究揭示了BNP/NPPB在心脏压力超负荷中的保护作用。实验中利用NPPB抗体进行组织定位和血浆浓度测定，证明BNP缺失会加剧心脏纤维化与功能障碍。
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**备注**：以上文献信息为示例性质，实际引用时请根据具体研究领域核实最新文献。建议通过PubMed或Web of Science以“NPPB antibody”或“BNP immunoassay”为关键词检索相关论文。
NPPB (Natriuretic Peptide Precursor B) antibody is a crucial tool in cardiovascular and renal research, targeting the protein product of the NPPB gene, which encodes B-type natriuretic peptide (BNP). BNP is a cardiac hormone primarily secreted by ventricular cardiomyocytes in response to myocardial stretch and pressure overload. It regulates blood pressure, fluid balance, and cardiovascular homeostasis through natriuresis, diuresis, and vasodilation via the guanylate cyclase-coupled natriuretic peptide receptor A (NPR-A). Elevated BNP levels serve as a clinical biomarker for heart failure and other cardiac pathologies.
The NPPB antibody is widely used in immunoassays (e.g., ELISA, Western blot, immunohistochemistry) to detect and quantify BNP expression in tissues, blood, or cultured cells. It aids in studying cardiac remodeling, hypertrophy, and fibrosis in both human samples and experimental models (e.g., rodents). Researchers also employ it to investigate BNP's roles in kidney function, metabolic disorders, and its counter-regulatory effects on the renin-angiotensin-aldosterone system. Species specificity (human, mouse, rat) and validation in knockout models are critical considerations for experimental accuracy. Proper validation ensures reliable detection of the 32-amino-acid active BNP peptide or its precursor forms, supporting translational research and diagnostic assay development.
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