| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | INT2; HBGF-3 |
| WB Predicted band size | 27 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Synthetic peptide of human FGF3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于FGF3抗体的参考文献示例(文献信息为示例性概括，实际引用时需核实准确性)：
1. **"Role of FGF3 in inner ear development: Insights from antibody-mediated localization"**
*Mansour, S.L., et al. (2005). Development.*
摘要：研究利用FGF3特异性抗体在小鼠模型中定位FGF3蛋白表达，揭示其在耳蜗和半规管发育中的关键作用，抗体用于免疫组化验证基因敲除模型的表型。
2. **"FGF3 overexpression in oral squamous cell carcinoma correlates with poor prognosis"**
*Yoshioka, Y., et al. (2012). Oral Oncology.*
摘要：通过抗FGF3抗体的免疫染色分析口腔鳞癌组织，发现FGF3高表达与肿瘤侵袭性和患者生存率降低相关，提示其作为潜在生物标志物的价值。
3. **"Monoclonal antibody targeting FGF3 inhibits tumor growth in xenograft models"**
*Smith, J.T., et al. (2018). Journal of Biological Chemistry.*
摘要：报道一种新型抗FGF3单克隆抗体的开发，该抗体通过阻断FGF3与受体结合抑制下游信号通路，在乳腺癌异种移植模型中显著降低肿瘤增殖。
4. **"FGF3 regulates zebrafish pharyngeal arch development via antibody-validated pathways"**
*Kiefer, P., et al. (2010). Developmental Biology.*
摘要：利用FGF3抗体进行斑马鱼胚胎蛋白表达分析，结合功能实验证明FGF3对咽弓形态发生的调控作用，抗体特异性通过Western blot验证。
**注意**：上述文献信息为示例性概括，实际引用时请通过PubMed、Google Scholar等数据库检索真实文献并核对细节。
Fibroblast Growth Factor 3 (FGF3) is a member of the FGF family, which comprises 22 structurally related proteins regulating cell proliferation, differentiation, migration, and survival. FGF3. initially identified as a proto-oncogene (INT2) in mouse mammary tumors, binds to FGF receptors (FGFRs) with heparan sulfate proteoglycans as cofactors. It plays critical roles in embryonic development, particularly in inner ear morphogenesis, tooth bud formation, and limb patterning. Dysregulation of FGF3 is linked to developmental disorders and cancers, such as breast, colorectal, and hepatocellular carcinomas, where it may drive tumor growth, angiogenesis, or metastasis.
FGF3 antibodies are essential tools for detecting and quantifying FGF3 expression in research and diagnostics. They are used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and ELISA to study FGF3's spatial-temporal expression, interactions, and signaling pathways. Polyclonal antibodies target multiple epitopes, offering high sensitivity, while monoclonal antibodies provide specificity for precise applications. These antibodies aid in exploring FGF3's role in disease mechanisms, evaluating its potential as a therapeutic target, or assessing drug efficacy in preclinical models. However, cross-reactivity with other FGF family members remains a challenge, necessitating rigorous validation using knockout controls or epitope mapping. Commercial FGF3 antibodies are typically raised in rabbits or mice, with recombinant or peptide antigens used for immunization. Proper sample preparation and antigen retrieval are critical for optimal performance, especially in FFPE tissues. Ongoing research continues to refine antibody specificity and expand their applications in both basic and translational studies.
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