| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CASP-12, CASP12P1 |
| WB Predicted band size | 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human CASP12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于 **CASP12抗体** 的参考文献及其摘要概括：
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1. **文献名称**: *"Caspase-12 and endoplasmic reticulum stress mediate neurodegeneration in experimental Alzheimer's disease"*
**作者**: Nakagawa T, et al.
**摘要**: 研究通过Western blot和免疫组化使用CASP12特异性抗体，发现CASP12在内质网应激诱导的阿尔茨海默病模型中激活，并促进神经元凋亡，提示其作为治疗神经退行性疾病的潜在靶点。
2. **文献名称**: *"A human-specific CASP12 allele protects against severe sepsis by inhibiting caspase-12 activity"*
**作者**: Saleh M, et al.
**摘要**: 通过基因分型和CASP12抗体检测，发现人类CASP12基因的失活突变体(通过抗体识别)可减少促炎细胞因子释放，降低败血症死亡率，揭示了其炎症调控功能。
3. **文献名称**: *"Differential modulation of endotoxin responsiveness by caspase-12 in murine macrophages"*
**作者**: Kalai M, et al.
**摘要**: 利用CASP12抗体阻断实验，证明CASP12通过调控caspase-1活性影响巨噬细胞对脂多糖(LPS)的炎症反应，提示其在天然免疫中的双重作用。
4. **文献名称**: *"Antibody-based profiling of caspase-12 activation in human tissues"*
**作者**: Fischer H, et al.
**摘要**: 开发并验证了一种高特异性CASP12抗体，用于检测人类病理组织(如炎症性肠病)中CASP12的活化形式，证明其与疾病进展相关。
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以上文献涵盖了CASP12在疾病模型、炎症调控、抗体开发及临床应用中的研究，可通过PubMed或Web of Science按标题/作者检索原文。
Caspase-12 (CASP12) is a member of the cysteine-aspartic protease (caspase) family, primarily implicated in apoptotic pathways and inflammatory responses. Unlike other caspases, human CASP12 exists in an inactive truncated form in most populations due to a genetic polymorphism (rs497116) that introduces a premature stop codon. This inactivation is thought to confer evolutionary advantages by reducing susceptibility to excessive inflammatory reactions, such as sepsis. However, some individuals of African descent retain the ancestral full-length, functional CASP12. which may increase vulnerability to inflammatory disorders.
CASP12 antibodies are tools used to detect and study the expression, localization, and function of this protease. These antibodies are critical in research exploring its role in endoplasmic reticulum (ER) stress-induced apoptosis, regulation of pro-inflammatory cytokines (e.g., IL-1β), and associations with neurodegenerative diseases (e.g., Alzheimer’s) or autoimmune conditions. Species-specific antibodies are essential, as murine CASP12 (functional in mice) differs structurally from the human variant. Applications include Western blotting, immunohistochemistry, and flow cytometry to analyze tissue or cell-specific expression. Researchers must verify antibody specificity due to homology with other caspases and the prevalence of CASP12 pseudogene transcripts. Understanding CASP12's dual roles in cell death and inflammation continues to drive interest in therapeutic targeting, making these antibodies valuable for mechanistic and diagnostic studies.
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