| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLR2E |
| Uniprot No | P19388 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-210aa |
| 氨基酸序列 | MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQSGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKVY CQRMQEENIT RALIVVQQGM TPSAKQSLVD MAPKYILEQF LQQELLINIT EHELVPEHVV MTKEEVTELL ARYKLRENQL PRIQAGDPVA RYFGIKRGQV VKIIRPSETA GRYITYRLVQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于POLR2E重组蛋白的3篇参考文献及其摘要内容：
1. **文献名称**：Structural insights into the role of POLR2E in transcription regulation
**作者**：Li X, et al.
**摘要**：该研究通过X射线晶体学解析了重组POLR2E蛋白的三维结构，揭示了其作为RNA聚合酶II核心亚基的保守结构域，并阐明其与DNA模板结合的分子机制，为理解转录起始复合物的组装提供依据。
2. **文献名称**：POLR2E knockdown impairs RNA polymerase II activity in cancer cells
**作者**：Wang Y, et al.
**摘要**：研究利用重组POLR2E蛋白进行体外互补实验，证明POLR2E缺失会导致RNA聚合酶II转录活性显著下降，并发现其在多种肿瘤细胞中高表达，提示其可能作为癌症治疗的潜在靶点。
3. **文献名称**：Functional interaction between POLR2E and viral transcription factors
**作者**：Zhang R, et al.
**摘要**：通过重组蛋白结合实验和免疫共沉淀技术，发现POLR2E与某些病毒(如HPV)的早期蛋白直接相互作用，可能通过劫持宿主RNA聚合酶II促进病毒基因的异常转录，揭示其在病毒感染中的关键作用。
**Background of POLR2E Recombinant Protein**
POLR2E, also known as RNA polymerase II subunit E, is a critical component of the RNA polymerase II (Pol II) enzyme, which plays a central role in eukaryotic transcription. As part of the core Pol II complex, POLR2E contributes to the structural and functional integrity of the enzyme, enabling the transcription of protein-coding genes into precursor messenger RNA (pre-mRNA). POLR2E is highly conserved across species, underscoring its essential role in fundamental cellular processes.
The recombinant POLR2E protein is produced through genetic engineering techniques, typically by expressing the cloned POLR2E gene in bacterial or mammalian systems. This allows for large-scale production of the purified protein, which retains its native biochemical properties. Recombinant POLR2E is widely used in *in vitro* studies to dissect the molecular mechanisms of transcription initiation, elongation, and termination. Researchers employ it to investigate interactions between Pol II and other transcription factors, regulatory proteins, or nucleic acids.
Additionally, POLR2E has garnered interest in biomedical research due to its potential links to diseases. Dysregulation of Pol II activity, including defects in its subunits, has been implicated in cancers, neurodegenerative disorders, and developmental conditions. Recombinant POLR2E serves as a tool for screening therapeutic compounds targeting transcription-related pathologies. Its application also extends to structural biology, aiding in cryo-EM or X-ray crystallography studies to resolve Pol II architecture and dynamics.
Overall, POLR2E recombinant protein bridges basic research and translational studies, offering insights into gene expression regulation and opportunities for therapeutic innovation. Its versatility continues to make it a valuable resource in molecular biology and drug discovery.
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