纯度 | >95%SDS-PAGE. |
种属 | Human |
靶点 | POLR2F |
Uniprot No | P61218 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-127aa |
氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSDNEDN FDGDDFDDVE EDEGLDDLEN AEEEGQENVE ILPSGERPQA NQKRITTPYM TKYERARVLG TRALQIAMCA PVMVELEGET DPLLIAMKEL KARKIPIIIR RYLPDGSYED WGVDELIITD |
预测分子量 | 17 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于POLR2F重组蛋白的3篇参考文献示例(注:文献信息为模拟示例,实际引用需核实原文):
1. **文献名称**:*Structural Insights into RNA Polymerase II Subunit Composition*
**作者**:Cramer P, et al.
**摘要**:该研究解析了人RNA聚合酶II的亚基组成及三维结构,通过重组表达并纯化POLR2F等亚基,揭示了其在转录起始复合体中的关键作用及与其他亚基的相互作用。
2. **文献名称**:*Functional Characterization of Recombinant POLR2F in Transcription Regulation*
**作者**:Hengartner CJ, et al.
**摘要**:利用重组POLR2F蛋白进行体外转录实验,发现其通过调节RNA聚合酶II的催化活性影响转录延伸效率,并验证了其在基因表达调控中的必要性。
3. **文献名称**:*Recombinant Expression and Interaction Mapping of POLR2F in Cancer Cells*
**作者**:Li Y, et al.
**摘要**:研究通过重组POLR2F蛋白的体外互作实验,结合质谱分析,鉴定了其与多种转录因子的结合网络,提示POLR2F异常表达可能与肿瘤发生相关。
如需具体文献,建议通过PubMed或Google Scholar搜索关键词“POLR2F recombinant protein”或“RNA polymerase II subunit F”获取最新研究。
POLR2F, also known as RNA polymerase II subunit F, is a critical component of the RNA polymerase II (Pol II) complex, the enzyme responsible for synthesizing messenger RNA (mRNA) in eukaryotic cells. Encoded by the POLR2F gene, this protein forms part of the core structure of Pol II, which consists of 12 subunits (RPB1-RPB12). Specifically, POLR2F corresponds to RPB6. a shared subunit found in all three RNA polymerases (I, II, III), highlighting its conserved role in transcription machinery. It contributes to polymerase stability and facilitates interactions with transcription factors during initiation and elongation phases.
Recombinant POLR2F protein is engineered for in vitro studies to dissect its structural and functional contributions to transcription regulation. Produced using expression systems like *E. coli* or mammalian cells, the recombinant protein often includes affinity tags (e.g., His-tag) for purification. Researchers utilize it to investigate Pol II assembly, promoter binding, and the impact of mutations or post-translational modifications on transcription. Its applications extend to drug discovery, particularly in targeting transcription dysregulation linked to cancers, neurodegenerative disorders, or viral infections that hijack host transcription. Additionally, POLR2F serves as a tool for studying diseases associated with RNA polymerase dysfunction, such as developmental disorders. By enabling precise biochemical and structural analyses, recombinant POLR2F advances our understanding of gene expression mechanisms and therapeutic interventions.
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