| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLR2H |
| Uniprot No | P52434 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-150aa |
| 氨基酸序列 | AGILFEDIF DVKDIDPEGK KFDRVSRLHC ESESFKMDLI LDVNIQIYPV DLGDKFRLVI ASTLYEDGTL DDGEYNPTDD RPSRADQFEY VMYGKVYRIE GDETSTEAAT RLSAYVSYGG LLMRLQGDAN NLHGFEVDSR VYLLMKKLAF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于POLR2H重组蛋白的3篇代表性文献，按研究方向和摘要内容分类整理：
1. **文献名称**：Structural insights into the RNA polymerase II core assembly
**作者**：Sadian Y, et al.
**摘要**：通过冷冻电镜技术解析了人源RNA聚合酶II核心复合体的高分辨率结构，揭示了POLR2H亚基在稳定核心模块(Rpb3-Rpb11-POLR2H)中的关键作用，并阐明了其重组表达对复合体组装的影响。
2. **文献名称**：Recombinant expression and functional analysis of POLR2H in transcription regulation
**作者**：Kwon I, et al.
**摘要**：成功构建了POLR2H的重组表达系统，发现该蛋白通过与转录因子TFIIF的相互作用调节RNA聚合酶II的启动子结合效率，为体外研究转录机制提供了工具。
3. **文献名称**：POLR2H mutations disrupt RNA polymerase II integrity in developmental disorders
**作者**：Dixon J, et al.
**摘要**：首次报道POLR2H基因突变导致RNA聚合酶II复合体稳定性下降，通过重组蛋白互补实验证实突变体对转录延伸过程的损害，揭示了其在神经发育异常中的分子机制。
*注：以上文献信息为模拟生成，实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词"POLR2H recombinant"、"RNA polymerase II subunit H"或结合疾病关联词(如"cancer"/"neurodevelopment")进行扩展检索。*
POLR2H, also known as RPB8. is a key subunit of RNA polymerase II (Pol II), the enzyme responsible for transcribing protein-coding genes in eukaryotes. As part of the catalytic core of Pol II, POLR2H plays a structural and functional role in maintaining the stability and activity of the polymerase complex. It interacts with other subunits like RPB1 and RPB6. contributing to the assembly of the enzyme and its ability to bind DNA templates during transcription initiation and elongation.
Recombinant POLR2H protein is produced using heterologous expression systems, such as *E. coli* or mammalian cell lines, enabling large-scale purification for biochemical and structural studies. Its recombinant form retains the ability to integrate into Pol II complexes, making it valuable for dissecting transcription mechanisms, including the regulation of gene expression and responses to cellular stress. Studies have highlighted its involvement in coordinating post-translational modifications of Pol II, such as phosphorylation, which modulate transcriptional pausing and termination.
Research on POLR2H also extends to disease contexts. Dysregulation of Pol II activity is linked to cancers, neurodegenerative disorders, and viral infections that hijack host transcription machinery. Recombinant POLR2H aids in screening for small-molecule inhibitors targeting transcription-driven pathologies. Additionally, structural analyses using recombinant protein have clarified how mutations in Pol II subunits disrupt transcription, offering insights into congenital developmental disorders.
Overall, POLR2H recombinant protein serves as a critical tool for exploring fundamental transcriptional processes and developing therapeutic strategies against diseases tied to transcriptional dysregulation. Its study bridges molecular biology, structural biology, and translational medicine.
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