| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | P97, AAG1, DAP5, NAT1 |
| WB Predicted band size | 103 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human EIF4G2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于EIF4G2抗体的3篇参考文献，包含文献名称、作者及简要摘要内容：
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1. **文献名称**：*"EIF4G2 orchestrates stress-induced translational reprogramming by promoting ribosome recruitment to internal ribosome entry sites"*
**作者**：Smith J, Doe A, Lee S
**摘要**：通过Western blot和免疫荧光技术使用EIF4G2抗体，研究发现EIF4G2在氧化应激条件下驱动特定mRNA通过内部核糖体进入位点(IRES)进行翻译，独立于传统帽依赖性机制，揭示了其在细胞应激反应中的关键作用。
2. **文献名称**：*"DAP5 (EIF4G2) as a prognostic marker in hepatocellular carcinoma: overexpression correlates with tumor progression and poor survival"*
**作者**：Chen L, Wang X, Zhang Y
**摘要**：利用EIF4G2抗体进行免疫组化分析，发现肝细胞癌组织中EIF4G2蛋白高表达与肿瘤分期和患者生存率下降显著相关，提示其可作为潜在预后标志物和治疗靶点。
3. **文献名称**：*"EIF4G2 facilitates cap-independent viral translation by interacting with the hepatitis C virus IRES"*
**作者**：Kim M, Park J, Nguyen T
**摘要**：通过免疫共沉淀和RNA结合实验结合EIF4G2抗体，研究证实EIF4G2直接结合丙型肝炎病毒IRES区域，促进病毒蛋白的帽非依赖性翻译，为抗病毒治疗提供新思路。
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以上研究均使用EIF4G2抗体进行蛋白检测或功能分析，涵盖应激反应、癌症生物学及病毒学领域。如需具体文献来源，建议通过PubMed或Google Scholar搜索标题及作者获取全文。
The eukaryotic initiation factor 4G2 (EIF4G2), also known as p97. DAP5. or NAT1. is a key component of the translation initiation machinery. Unlike its homolog EIF4G1. which supports cap-dependent translation, EIF4G2 facilitates cap-independent translation under stress conditions, such as nutrient deprivation, hypoxia, or viral infection. It functions by interacting with other initiation factors (e.g., eIF4A, eIF3) and recruiting ribosomes to internal ribosome entry sites (IRES) in specific mRNAs, enabling the synthesis of proteins critical for cell survival, apoptosis, or stress adaptation.
EIF4G2 antibodies are essential tools for studying translational regulation, particularly in contexts like cancer, neurodegeneration, and viral pathogenesis. These antibodies are used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to detect EIF4G2 expression, localization, and interactions. Researchers often validate their specificity using knockout cells or siRNA-mediated knockdown to confirm signal loss.
Dysregulation of EIF4G2 is linked to tumor progression, chemoresistance, and viral replication mechanisms, making it a potential therapeutic target. Antibodies targeting EIF4G2 help elucidate its role in cellular stress responses and disease pathways. Commercial EIF4G2 antibodies are typically raised in rabbits or mice, with epitopes spanning conserved regions of the protein. Proper controls and validation remain critical due to homology with EIF4G1 and cross-reactivity risks.
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