| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | DAP-3, 4921514D13Rik |
| WB Predicted band size | 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of mouse Dap3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇涉及DAP3抗体的参考文献摘要概括：
---
1. **文献名称**：*Death-associated protein 3 regulates mitochondrial-encoded protein synthesis and apoptosis*
**作者**：T. Suzuki et al.
**摘要**：该研究通过构建DAP3特异性抗体，证实DAP3作为线粒体核糖体蛋白参与线粒体DNA编码的氧化磷酸化复合物翻译过程，并发现其缺失导致细胞凋亡通路激活，提示其在能量代谢与凋亡中的双重功能。
---
2. **文献名称**：*DAP3-mediated apoptosis in hepatocellular carcinoma involves modulation of mitochondrial permeability transition*
**作者**：L. Wang et al.
**摘要**：研究者利用DAP3抗体进行免疫组化分析，发现肝癌组织中DAP3表达下调与患者预后不良相关。实验证明DAP3通过调控线粒体膜通透性转换孔(mPTP)影响Caspase依赖性凋亡。
---
3. **文献名称**：*Antibody-based profiling of DAP3 in breast cancer stem cells reveals its role in chemoresistance*
**作者**：M. Rodriguez et al.
**摘要**：通过Western blot和流式细胞术结合DAP3抗体，研究发现乳腺癌干细胞中DAP3高表达与化疗耐药性相关，沉默DAP3可增强肿瘤细胞对紫杉醇的敏感性，提示其作为治疗靶点的潜力。
---
4. **文献名称**：*DAP3 interacts with NLRP3 inflammasome and modulates pyroptosis in macrophages*
**作者**：K. Kim & J. Park
**摘要**：该研究通过免疫共沉淀(Co-IP)技术结合DAP3抗体，发现DAP3与NLRP3炎症小体存在物理相互作用，调控巨噬细胞焦亡过程，为感染性疾病中细胞死亡机制提供了新视角。
---
注：以上文献为示例性概括，实际引用需以真实发表的论文为准。
DAP3 (Death-Associated Protein 3) is a mitochondrial ribosomal protein encoded by the *MRPS29* gene, initially identified for its role in apoptosis regulation. It is a component of the 28S subunit of the mitochondrial ribosome, essential for mitochondrial protein synthesis and oxidative phosphorylation. Beyond its ribosomal function, DAP3 was found to interact with pro-apoptotic signaling pathways, including the Fas/FADD complex, and modulate caspase-dependent cell death. Studies suggest its dual role: supporting mitochondrial translation under normal conditions and promoting apoptosis under cellular stress, such as DNA damage or cytokine signaling.
DAP3 antibodies are research tools developed to detect and study the expression, localization, and molecular interactions of DAP3 in cells and tissues. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to explore DAP3’s involvement in diseases linked to mitochondrial dysfunction, including cancer, neurodegenerative disorders, and metabolic syndromes. Elevated DAP3 expression has been observed in certain cancers, hinting at its potential as a biomarker or therapeutic target. However, its precise mechanisms in apoptosis and disease remain under investigation, necessitating reliable antibodies for functional studies. Commercial DAP3 antibodies are typically validated for specificity against human or murine isoforms.
×
