| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | JM2, AIID, IPEX, PIDX, XPID, DIETER |
| WB Predicted band size | 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human FOXP3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于FOXP3抗体的3篇代表性文献及其摘要：
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1. **"Control of regulatory T cell development by the transcription factor Foxp3"**
*Fontenot, J.D., et al. (Nature Immunology, 2003)*
该研究首次开发了针对FOXP3的单克隆抗体，证实FOXP3是调节性T细胞(Treg)的关键转录因子。通过抗体染色和功能实验，证明FOXP3缺失导致Treg功能缺陷和自身免疫疾病。
2. **"Foxp3 programs the development and function of CD4+CD25+ regulatory T cells"**
*Hori, S., et al. (Science, 2003)*
作者利用FOXP3特异性抗体，揭示其在Treg发育中的核心作用。研究表明，FOXP3的过表达可诱导naive T细胞获得抑制功能，而抗体染色证实其特异性地在Treg中表达。
3. **"Regulatory T cells and immune tolerance"**
*Sakaguchi, S., et al. (Annual Review of Immunology, 2008)*
这篇综述总结了FOXP3作为Treg标志物的研究进展，强调了FOXP3抗体在临床和实验中的应用，包括通过流式细胞术或免疫组化检测Treg分布及功能。
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这些文献涵盖了FOXP3抗体的开发、功能验证及在基础与临床研究中的关键应用。
FOXP3 antibodies are essential tools in immunological research, primarily used to identify and study regulatory T cells (Tregs), a subset of CD4+ T cells critical for maintaining immune tolerance and preventing autoimmune diseases. The FOXP3 protein, encoded by the *FOXP3* gene, is a transcription factor belonging to the forkhead box family. It serves as a master regulator of Treg development and function, with mutations in *FOXP3* linked to severe autoimmune disorders like IPEX syndrome (immune dysregulation, polyendocrinopathy, enteropathy, X-linked).
FOXP3 antibodies target specific epitopes of the FOXP3 protein, enabling detection in various experimental applications, including flow cytometry, immunohistochemistry, and Western blotting. These antibodies help distinguish Tregs from other T-cell populations, as FOXP3 is predominantly expressed in Tregs. However, its expression can vary depending on activation status, posing challenges in accurate quantification. Researchers also use FOXP3 antibodies to explore Treg dynamics in cancer, transplantation, and autoimmune conditions, where Treg dysfunction or depletion is often implicated.
Commercial FOXP3 antibodies are typically raised against human or murine FOXP3. with clones like 236A/E7 (human) and FJK-16s (mouse) widely validated. Despite their utility, variability in antibody specificity and cross-reactivity necessitates careful validation. Recent advances in single-cell technologies and multiplex assays have further highlighted FOXP3’s role in immune modulation, reinforcing the importance of reliable FOXP3 antibodies in both basic research and clinical diagnostics.
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