首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠封闭蛋白5(CLDN5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CLDN5含量。CLDN5即闭合蛋白5，是紧密连接蛋白家族成员，在血管内皮细胞广泛表达，对维持血脑屏障完整性至关重要。研究发现其可作为肿瘤治疗靶点，通过调节其表达或功能，影响肿瘤血管生成和转移，为肿瘤诊断与治疗提供新思路。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，本试剂盒通过特异性捕获抗体与检测抗体的协同作用，可实现目标蛋白的高效识别与定量分析，适用于生命科学研究中探索CLDN5在组织屏障功能调控、疾病模型建立、药物干预效果评估等领域的表达变化规律。为研究者提供了一种稳定、可靠的检测工具，适用于体外实验模型中CLDN5蛋白水平的动态监测及相关分子机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cldn5; Bec1; Claudin-5; Brain endothelial cell clone 1 protein; Lung-specific membrane protein
缩写：CLDN5
Uniprot No.：O54942
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLDN5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 101 | |||||
| Range % | 95-104 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 90-99 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 86-97 | ||||||
回收率：
| The recovery of mouse CLDN5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 88 | 83-92 | |||||
| EDTA plasma (n=4) | 93 | 87-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected12002.3122.4222.3672.2406001.8461.9661.9061.7793001.2611.2291.2451.1181500.7540.7690.7620.635750.4430.4560.4500.32337.50.2710.2810.2760.14918.750.2080.2020.2050.07800.1260.1280.127 | |||||||
货期：3-5 working days
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