| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human GSK3B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GSK3B抗体的3篇参考文献的简要概括：
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1. **文献名称**: "GSK3β regulates differentiation and growth arrest in glioblastoma"
**作者**: Patel et al.
**摘要**: 该研究利用GSK3B特异性抗体(克隆号：3D10)通过Western blot和免疫荧光分析胶质母细胞瘤细胞中的GSK3B蛋白表达及磷酸化状态，揭示了GSK3B在肿瘤细胞分化中的调控作用。
2. **文献名称**: "A novel role for GSK3β in glucose homeostasis through insulin signaling"
**作者**: Zhang et al.
**摘要**: 作者使用兔源多克隆GSK3B抗体(货号：ab93926)进行免疫沉淀和免疫组化实验，证明GSK3B在胰岛素信号通路中通过磷酸化调控糖代谢，抗体特异性经siRNA敲低验证。
3. **文献名称**: "Phospho-specific antibodies reveal spatiotemporal activation of GSK3β in Alzheimer's disease models"
**作者**: Hernandez et al.
**摘要**: 研究开发了针对GSK3B磷酸化位点(Ser9)的小鼠单克隆抗体，通过脑组织切片染色和蛋白质印迹验证其在阿尔茨海默病模型中活性变化，表明磷酸化GSK3B与tau蛋白异常聚集相关。
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提示：具体实验需根据抗体来源(如CST、Abcam等厂商)及克隆号进一步筛选验证文献。
Glycogen synthase kinase 3 beta (GSK3B) is a ubiquitously expressed serine/threonine protein kinase involved in diverse cellular processes, including metabolism, cell proliferation, differentiation, and apoptosis. Unlike most kinases, GSK3B is constitutively active under basal conditions and primarily regulated through inhibitory phosphorylation at Ser9 (by AKT, PKC, etc.) or activation via Tyr216 phosphorylation. It functions as a critical node in multiple signaling pathways, such as Wnt/β-catenin, insulin, and PI3K/AKT cascades, impacting both physiological and pathological contexts, such as neurodegenerative diseases, diabetes, and cancer.
GSK3B antibodies are essential tools for studying its expression, localization, and activity in research. They enable detection of total GSK3B protein or specific phosphorylation states (e.g., pSer9 or pTyr216) using techniques like Western blotting, immunohistochemistry, or immunofluorescence. These antibodies help elucidate GSK3B's role in disease mechanisms, such as tau hyperphosphorylation in Alzheimer’s disease or insulin resistance in type 2 diabetes. Additionally, they are used to evaluate therapeutic interventions targeting GSK3B, including small-molecule inhibitors (e.g., lithium, CHIR99021). Due to its structural homology with GSK3A, antibody specificity validation is crucial to avoid cross-reactivity. Research-grade antibodies often target unique epitopes within the N-terminal or C-terminal domains to ensure precise detection. Understanding GSK3B regulation through these antibodies continues to drive insights into its dual roles as a tumor suppressor or promoter, depending on cellular context.
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